Application | Comment | Organism |
---|---|---|
synthesis | the highly thermostable enzyme constitutes a useful catalyst for a simplified synthesis of (4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid)-(1->4)-(alpha-D-galactopyranosyluronic acid)-(1->4)-alpha-D-galactopyranuronic acid which is extremely difficult to obtain via chemical synthesis | Thermotoga maritima |
Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EGTA | - |
Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9WYR4 | - |
- |
Thermotoga maritima DSM 3109 | Q9WYR4 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | precursor undergoes N-terminal processing by cleavage at a putative site between alanine and serine residues | Thermotoga maritima |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
polygalacturonic acid | the enzyme catalyzes selectively a x01-4,5 elimination at the third galacturonic unit from the reducing end of polygalacturonic acid by producing (4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid)-(1->4)-(alpha-D-galactopyranosyluronic acid)-(1->4)-alpha-D-galactopyranuronic acid with a 60% yield | Thermotoga maritima | (4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid)-(1->4)-(alpha-D-galactopyranosyluronic acid)-(1->4)-alpha-D-galactopyranuronic acid | - |
? | |
polygalacturonic acid | the enzyme catalyzes selectively a x01-4,5 elimination at the third galacturonic unit from the reducing end of polygalacturonic acid by producing (4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid)-(1->4)-(alpha-D-galactopyranosyluronic acid)-(1->4)-alpha-D-galactopyranuronic acid with a 60% yield | Thermotoga maritima DSM 3109 | (4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid)-(1->4)-(alpha-D-galactopyranosyluronic acid)-(1->4)-alpha-D-galactopyranuronic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
Pel | - |
Thermotoga maritima |
Tm0433 | - |
Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
the enzyme loses 50% of its initial activity within 30 min | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Thermotoga maritima |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 10 | pH 8.5: about 70% of maximal activity, pH 10.0: about 50% of maximal activity. Below pH 7 or above pH 11, no appreciable activity can be detected | Thermotoga maritima |