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Literature summary for 4.2.2.21 extracted from
- Chondroitinase ABC I from Proteus vulgaris: cloning, recombinant expression and active site identification (2005), Biochem. J., 386, 103-112.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | optimal concentration 62.5 mM for substrate chondroitin 6-sulfate, inhibitory above 125 mM, optimal concentration 125 mM for substrate dermatan sulfate, inhibitory above 250 mM | Proteus vulgaris |  |
| Sodium acetate | required | Proteus vulgaris | |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E653A | no enzymic activity | Proteus vulgaris |
| H501A | no enzymic activity | Proteus vulgaris |
| H561A | increase in Km-values | Proteus vulgaris |
| H712A | increase in Km-values | Proteus vulgaris |
| R500A | increase in Km-values, large decrease in kcat values | Proteus vulgaris |
| R560A | no enzymic activity | Proteus vulgaris |
| Y508A | no enzymic activity | Proteus vulgaris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | optimal concentration 62.5 mM for substrate chondroitin 6-sulfate, 50% inhibition at 125 mM, optimal concentration 125 mM for substrate dermatan sulfate, 50% inhibition at 250 mM | Proteus vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0012 | - |
chondroitin 6-sulfate | wild-type, pH 8.0 | Proteus vulgaris | |
| 0.0015 | - |
chondroitin 4-sulfate | wild-type, pH 8.0 | Proteus vulgaris | |
| 0.0025 | - |
dermatan sulfate | wild-type, pH 8.0 | Proteus vulgaris | |
| 0.0051 | - |
dermatan sulfate | mutant H712A, pH 8.0 | Proteus vulgaris | |
| 0.0069 | - |
dermatan sulfate | mutant H561A, pH 8.0 | Proteus vulgaris | |
| 0.0086 | - |
chondroitin 6-sulfate | mutant H712A, pH 8.0 | Proteus vulgaris | |
| 0.015 | - |
chondroitin 6-sulfate | mutant H561A, pH 8.0 | Proteus vulgaris | |
| 0.02 | - |
chondroitin 6-sulfate | mutant R500A, pH 8.0 | Proteus vulgaris | |
| 0.036 | - |
dermatan sulfate | mutant R500A, pH 8.0 | Proteus vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
x * 110000, SDS-PAGE, x * 112614, calculated | Proteus vulgaris |
| 112614 | - |
x * 110000, SDS-PAGE, x * 112614, calculated | Proteus vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Proteus vulgaris | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.8 | - |
substrate hyaluronan, pH 8.0 | Proteus vulgaris |
| 34.8 | - |
substrate chondroitin sulfate E, pH 8.0 | Proteus vulgaris |
| 54.2 | - |
substrate chondroitin sulfate D, pH 8.0 | Proteus vulgaris |
| 69.8 | - |
substrate chondroitin, pH 8.0 | Proteus vulgaris |
| 122.3 | - |
substrate dermatan sulfate, pH 8.0 | Proteus vulgaris |
| 174.6 | - |
substrate chondroitin 6-sulfate, pH 8.0 | Proteus vulgaris |
| 290.6 | - |
substrate chondroitin 4-sulfate, pH 8.0 | Proteus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chondroitin | - |
Proteus vulgaris | ? | - |
? | |
| chondroitin 6-sulfate | - |
Proteus vulgaris | unsaturated chondroitin 6-sulfate disaccharide | - |
? | |
| chondroitin sulfate D | - |
Proteus vulgaris | ? | - |
? | |
| chondroitin sulfate E | - |
Proteus vulgaris | ? | - |
? | |
| dermatan sulfate | - |
Proteus vulgaris | ? | - |
? | |
| hyaluronan | - |
Proteus vulgaris | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 110000, SDS-PAGE, x * 112614, calculated | Proteus vulgaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
and up to 37, substrate dermatan sulfate | Proteus vulgaris |
| 37 | - |
substrate chondroitin 6-sulfate | Proteus vulgaris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
dramatic loss of activity | Proteus vulgaris |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.7 | - |
dermatan sulfate | mutant R500A, pH 8.0 | Proteus vulgaris | |
| 7 | - |
chondroitin 6-sulfate | mutant R500A, pH 8.0 | Proteus vulgaris | |
| 10 | - |
dermatan sulfate | mutant H712A, pH 8.0 | Proteus vulgaris | |
| 19 | - |
chondroitin 6-sulfate | mutant H712A, pH 8.0 | Proteus vulgaris | |
| 66 | - |
dermatan sulfate | mutant H561A, pH 8.0 | Proteus vulgaris | |
| 450 | - |
dermatan sulfate | wild-type, pH 8.0 | Proteus vulgaris | |
| 617 | - |
chondroitin 6-sulfate | wild-type, pH 8.0 | Proteus vulgaris | |
| 652 | - |
chondroitin 6-sulfate | mutant H561A, pH 8.0 | Proteus vulgaris | |
| 867 | - |
chondroitin 4-sulfate | wild-type, pH 8.0 | Proteus vulgaris | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Proteus vulgaris |
| 9 | - |
inactive | Proteus vulgaris |
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