Literature summary for 4.2.2.20 extracted from

Nazari-Robati, M.; Khajeh, K.; Aminian, M.; Mollania, N.; Golestani, A.
Enhancement of thermal stability of chondroitinase ABC I by site-directed mutagenesis: an insight from Ramachandran plot (2013), Biochim. Biophys. Acta, 1834, 479-486.

Search for more literature for 4.2.2.20

Protein Variants

Protein Variants	Comment	Organism
Q140A	mutation improves both activity and thermal stability of the enzyme, and additionally improves resistance to trypsin degradation	Proteus vulgaris
Q140G	mutation improves both activity and thermal stability of the enzyme, and additionally improves resistance to trypsin degradation	Proteus vulgaris
Q140N	mutation reduces the enzyme activity and destabilizes	Proteus vulgaris

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.031	-	chondroitin 4-sulfate	mutant Q140A, pH 6.8, 25°C	Proteus vulgaris
0.034	-	chondroitin 4-sulfate	mutant Q140G, pH 6.8, 25°C	Proteus vulgaris
0.042	-	chondroitin 4-sulfate	wild-type, pH 6.8, 25°C	Proteus vulgaris
0.05	-	chondroitin 4-sulfate	mutant Q140N, pH 6.8, 25°C	Proteus vulgaris

Organism

Organism	UniProt	Comment	Textmining
Proteus vulgaris	P59807	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
chondroitin 4-sulfate	-	Proteus vulgaris	?	-	?

Synonyms

Synonyms	Comment	Organism
chondroitinase ABC I	-	Proteus vulgaris

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
80	-	chondroitin 4-sulfate	mutant Q140N, pH 6.8, 25°C	Proteus vulgaris
83	-	chondroitin 4-sulfate	wild-type, pH 6.8, 25°C	Proteus vulgaris
88	-	chondroitin 4-sulfate	mutant Q140A, pH 6.8, 25°C	Proteus vulgaris
89	-	chondroitin 4-sulfate	mutant Q140G, pH 6.8, 25°C	Proteus vulgaris

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1600	-	chondroitin 4-sulfate	mutant Q140N, pH 6.8, 25°C	Proteus vulgaris
2000	-	chondroitin 4-sulfate	wild-type, pH 6.8, 25°C	Proteus vulgaris
2600	-	chondroitin 4-sulfate	mutant Q140G, pH 6.8, 25°C	Proteus vulgaris
2800	-	chondroitin 4-sulfate	mutant Q140A, pH 6.8, 25°C	Proteus vulgaris

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Release 2023.1 (January 2023)