Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, 77% of initial activity | Bacillus subtilis | |
EDTA | 1 mM, 17% of initial activity | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
Polygalacturonate | Km value 0.312 mg/ml, pH 9.4, 50°C | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus subtilis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 1 mM, 139% of initial activity | Bacillus subtilis | |
Ca2+ | 1 mM, 257% of initial activity | Bacillus subtilis | |
Mg2+ | 1 mM, 144% of initial activity | Bacillus subtilis | |
Mn2+ | 1 mM, 143% of initial activity | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | A0A286K338 | - |
- |
Bacillus subtilis PB1 | A0A286K338 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1252 | - |
pH 9.4, 50°C | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
citrus pectin | - |
Bacillus subtilis | unsaturated oligogalacturonate | - |
? | |
citrus pectin | - |
Bacillus subtilis PB1 | unsaturated oligogalacturonate | - |
? | |
polygalacturonate | - |
Bacillus subtilis | unsaturated oligogalacturonides | - |
? | |
polygalacturonate | - |
Bacillus subtilis PB1 | unsaturated oligogalacturonides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43100, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | 2 h, 90% residual activity | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2300 | - |
Polygalacturonate | pH 9.4, 50°C | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | 2 h, 80% residual activity | Bacillus subtilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
Polygalacturonate | kcat/Km value 7372 ml/mg/s, pH 9.4, 50°C | Bacillus subtilis |