Cloned (Comment) | Organism |
---|---|
gene pl-str, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3), subcloning in Escherichia coli strain JM109 | Streptomyces sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 8% activation at 1 mM, 78.5% inhibition at 5 mM | Streptomyces sp. | |
Cr3+ | inhibits completely at 1 mM | Streptomyces sp. | |
Cu2+ | 11% activation at 1 mM, 41% inhibition at 5 mM | Streptomyces sp. | |
EDTA | inhibits completely at 1 mM | Streptomyces sp. | |
Fe3+ | 4% inhibition at 1 mM, 99% inhibition at 5 mM | Streptomyces sp. | |
Li+ | 5% activation at 1 mM, 62% inhibition at 5 mM | Streptomyces sp. | |
Mg2+ | 9% activation at 1 mM, 22% inhibition at 5 mM | Streptomyces sp. | |
Ni2+ | 4% inhibition at 1 mM, 93% inhibition at 5 mM | Streptomyces sp. | |
Pb2+ | 31% inhibition at 1 mM, complete inhibition at 5 mM | Streptomyces sp. | |
Zn2+ | 13% activation at 1 mM, 46% inhibition at 5 mM | Streptomyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required, optimal at about 0.05 mM | Streptomyces sp. | |
K+ | 9% activation at 1 mM | Streptomyces sp. | |
Mn2+ | 29% activation at 1 mM, 7.5% activation at 5 mM | Streptomyces sp. | |
Na+ | 10% activation at 1 mM | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33500 | - |
x * 33500, about, sequence calculation, x * 35000, recombinant enzyme, SDS-PAGE | Streptomyces sp. |
35000 | - |
x * 33500, about, sequence calculation, x * 35000, recombinant enzyme, SDS-PAGE | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
isolated from soil of Flaming Mountain in the Turpan Basin of Xinjiang, China, gene pl-str | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatgraphy | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
citrus pectin P9311 | - |
Streptomyces sp. | ? | - |
? | |
citrus pectin P9436 | - |
Streptomyces sp. | ? | - |
? | |
citrus pectin P9561 | - |
Streptomyces sp. | ? | - |
? | |
polygalacturonic acid | - |
Streptomyces sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33500, about, sequence calculation, x * 35000, recombinant enzyme, SDS-PAGE | Streptomyces sp. |
Synonyms | Comment | Organism |
---|---|---|
PL-STR | - |
Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Streptomyces sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
purified recombinant His-tagged enzyme, 1 h, without substrate, pH 12.0, over 58% activity remaining | Streptomyces sp. |
37 | - |
purified recombinant His-tagged enzyme, 1 h, without substrate, pH 7.0-10.0, over 80% activity remaining | Streptomyces sp. |
37 | 50 | purified recombinant His-tagged enzyme, 45 min, without substrate, pH 9.0-12.0, over 80% activity remaining | Streptomyces sp. |
50 | - |
purified recombinant His-tagged enzyme, 45 min, without substrate, pH 10.0, over 80% activity remaining | Streptomyces sp. |
60 | - |
purified recombinant His-tagged enzyme, 45 min, without substrate, pH 10.0, 30% activity remaining | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Streptomyces sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | purified recombinant His-tagged enzyme, 1 h, 60°C, without substrate, 30% activity remaining, at 37-50°C over 80% activity remain | Streptomyces sp. |
9 | 12 | purified recombinant His-tagged enzyme, 1 h, 60°C, without substrate, 30% activity remaining, at 37-50°C over 80% activity remain | Streptomyces sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptomyces sp. | sequence calculation | - |
6.83 |
General Information | Comment | Organism |
---|---|---|
evolution | PL-STR belongs to family PF00544 | Streptomyces sp. |