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Literature summary for 4.2.2.2 extracted from
- The alkaline pectate lyase PEL168 of Bacillus subtilis heterologously expressed in Pichia pastoris is more stable and efficient for degumming ramie fiber (2013), BMC Biotechnol., 13, 26.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DMSO | 24% activation at 0.5% | Bacillus subtilis |  |
| additional information | no or poor effect by Tween-80, methanol, ethanol, isopropyl alcohol at 0.5% | Bacillus subtilis |
Application
| Application | Comment | Organism |
|---|---|---|
| environmental protection | the conventional degumming process of ramie with alkaline treatment at high temperature causes severe environmental pollution. Pectate lyases can be used to remove pectin from ramie in a degumming process with reduced environmental pollution and energy consumption | Bacillus subtilis |
| industry | the conventional degumming process of ramie with alkaline treatment at high temperature causes severe environmental pollution. Pectate lyases can be used to remove pectin from ramie in a degumming process with reduced environmental pollution and energy consumption | Bacillus subtilis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pel168, enzyme expression in Pichia pastoris strain and in Escherichia coli, the latter shows less glycosylation and thermostability | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 53% inhibition at 5 mM | Bacillus subtilis | |
| Cu2+ | 93% inhibition at 5 mM | Bacillus subtilis | |
| EDTA | complete inhibition at 5 mM | Bacillus subtilis | |
| Fe3+ | 41% inhibition at 5 mM | Bacillus subtilis | |
| Hg2+ | complete inhibition at 5 mM | Bacillus subtilis | |
| Mg2+ | 64.5% inhibition at 5 mM | Bacillus subtilis | |
| Mn2+ | 91.5% inhibition at 5 mM | Bacillus subtilis | |
| additional information | no or poor effect by Tween-80, methanol, ethanol, isopropyl alcohol at 0.5% | Bacillus subtilis | |
| Ni2+ | 18% inhibition at 5 mM | Bacillus subtilis | |
| SDS | complete inhibition at 0.5% | Bacillus subtilis | |
| Zn2+ | 63.5% inhibition at 5 mM | Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required, activates the recombinant enzyme from Pichia pastoris by 26% | Bacillus subtilis | |
| additional information | no or poor effect on the recombinant enzyme from Pichia pastoris by Sr2+, K+, and Li+ | Bacillus subtilis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
x * 48600-51400, recombinant enzyme from Pichia pastoris, SDS-PAGE, x * 46000, recombinant enzyme from Escherichia coli, SDS-PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | P39116 | gene pel168 | - |
| Bacillus subtilis 168 | P39116 | gene pel168 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme has five putative N-glycosylation sites (Asn-X-Thr/Ser), three near to the N-terminus (N-48, N-127, N-184), and the other two near the C-terminus (N-345, N-351) | Bacillus subtilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1320 | - |
purified recombinant enzyme from Pichia pastoris, pH 9.5, 50°C | Bacillus subtilis |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48600-51400, recombinant enzyme from Pichia pastoris, SDS-PAGE, x * 46000, recombinant enzyme from Escherichia coli, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alkaline pectate lyase | - |
Bacillus subtilis |
| PEL168 | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified enzyme, 20 min, recombinant enzyme from Pichia pastoris retaines 26% activity, recombinant enzyme from Escherichia coli is inactivated | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Bacillus subtilis |
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