Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activity of pectate lyase is 9fold higher at 90°C in the presence of hydroxyapatite nanoparticles than in the presence of 1 mM CaCl2 | Priestia megaterium |
Protein Variants | Comment | Organism |
---|---|---|
additional information | improvement of thermostability and activity of pectate lyase in the presence of hydroxyapatite nanoparticles | Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | enzyme kinetics and thermodynamics at 75°C and 90°C in presence or absence of hydroxyapatite nanoparticles, detailed overview | Priestia megaterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates, optimal at 1 mM | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Priestia megaterium AK2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 18.6fold from strain AK2 culture supernatant by cation exchange chromatography and gel filtration | Priestia megaterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
852 | - |
purified native enzyme, pH 8.5, 55°C | Priestia megaterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Priestia megaterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
improvement of thermostability and activity of pectate lyase in the presence of hydroxyapatite nanoparticles | Priestia megaterium |
90 | - |
half-life of pectate lyase is 60fold higher in the presence of hydroxyapatite nanoparticles than in the presence of 1 mM CaCl2. Thermodynamic analysis of the nanoparticle-induced stability reveals an enhanced entropy-enthalpy compensation by hydroxyapatite nanoparticles since a reciprocal linearity of the enthalpy-entropy change to 90°C is observed | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Priestia megaterium |