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Literature summary for 4.2.2.2 extracted from

  • Payasi, A.; Misra, P.C.; Sanwal, G.G.
    Purification and characterization of pectate lyase from banana (Musa acuminata) fruits (2006), Phytochemistry, 67, 861-869.
    View publication on PubMed
Search for more literature for 4.2.2.2

Activating Compound

Activating Compound Comment Organism Structure
cystine 25% activation at 1 mM, 73% activation at 5 mM Musa acuminata

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 29% loss of activity at 1 mM, 67% loss of activity at 5 mM Musa acuminata
ascorbic acid 48% loss of activity at 0.1 mM, complete inactivation at 1.0 mM Musa acuminata
caffeic acid 42% inhibition at 0.01 mM, 67% inhibition at 0.05 mM Musa acuminata
catechol 24% inhibition at 0.01 mM, 52% inhibition at 0.05 mM Musa acuminata
chlorogenic acid 27% inhibition at 0.01 mM, 68% inhibition at 0.05 mM Musa acuminata
Cinnamic acid 40% inhibition at 0.01 mM, 67% inhibition at 0.05 mM Musa acuminata
dithiothreitol 27% loss of activity at 1 mM, 67% loss of activity at 5 mM Musa acuminata
ferulic acid 15% inhibition at 0.01 mM, 58% inhibition at 0.05 mM Musa acuminata
HgCl2 complete inactivation at 0.1 mM Musa acuminata
p-coumaric acid 33% inhibition at 0.01 mM, 61% inhibition at 0.05 mM Musa acuminata
PCMB 49% loss of activity at 0.01 mM, complete inactivation at 0.1 mM Musa acuminata
salicylic acid 33% inhibition at 0.01 mM, 61% inhibition at 0.05 mM Musa acuminata
Sodium metabisulfite 40% inactivation at 0.1 mM, 73% inactivation at 1 mM Musa acuminata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information KM-value for polygalacturonate is 0.4 g/l in presence of 0.7 mM Ca2+, KM-value for polygalacturonate is 0.32 g/l in presence of 0.7 mM Mg2+, KM-value for polygalacturonate is 0.45 g/l in presence of 0.7 mM Ca2+ + 0.7 mM Mg2+, KM-value for citrus pectin (7.2% methylation) is 0.77 g/l Musa acuminata

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required, can be replaced by Mn2+ or Mg2+. Optimal activity at 0.7 mM. Additive effect when any two metal ions (Mg2+, Ca2+, Mn2+) are present together Musa acuminata
Mg2+ can substitute for Ca2+ in activation. Optimal activity at 0.7 mM. Additive effect when any two metal ions (Mg2+, Ca2+, Mn2+) are present together Musa acuminata
Mn2+ can substitute for Ca2+ in activation. Optimal activity at 0.7 mM. Additive effect when any two metal ions (Mg2+, Ca2+, Mn2+) are present together Musa acuminata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 1 * 43000, SDS-PAGE Musa acuminata
44000
-
 gel filtration Musa acuminata

Organism

Organism UniProt Comment Textmining
Musa acuminata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Musa acuminata

Source Tissue

Source Tissue Comment Organism Textmining
fruit ripe Musa acuminata
-

Storage Stability

Storage Stability Organism
2-5°C, pH 7.0, 0.02 M sodium phosphate buffer, stable for at least 15 days Musa acuminata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl esterified pectin citrus pectin, 7% methylation Musa acuminata ?
-
 ?
polygalacturonate
-
 Musa acuminata unsaturated galacturonic acid polymer
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 43000, SDS-PAGE Musa acuminata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Musa acuminata

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 50 37°C: optimum, 45°C: 70% of maximal activity, 50°C: 55% of maximal activity Musa acuminata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 room temperature, activity is lost within 2 days Musa acuminata
40
-
 5 min, 5-10% loss of activity Musa acuminata
80
-
 5 min, complete inactivation Musa acuminata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Musa acuminata

pH Range

pH Minimum pH Maximum Comment Organism
7 10 pH 7.0: about 40% of maximal activity, pH 10.0: about 60% of maximal activity Musa acuminata

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 30% loss of activity after 3 h, 90% loss of activity after 24 h Musa acuminata
7 8.5 stable Musa acuminata

pI Value

Organism Comment pI Value Maximum pI Value
Musa acuminata isoelectric focusing, pH range 3-10
-
 8