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Literature summary for 4.2.2.2 extracted from

  • Walker, S.G.; Ryan, M.E.
    Cloning and expression of a pectate lyase from the oral spirochete Treponema pectinovorum ATCC 33768 (2003), FEMS Microbiol. Lett., 226, 385-390.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Treponema pectinovorum

Inhibitors

Inhibitors Comment Organism Structure
EDTA 3 mM, complete inhibition. Lyase activity can be restored by further addition of 5 mM CaCl2 Treponema pectinovorum
MnSO4 0.1 mM, 10% decrease in activity Treponema pectinovorum
ZnSO4 0.1 mM, 90% decrease in activity Treponema pectinovorum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ requires Ca2+, optimal activity with 0.25 mM Treponema pectinovorum

Organism

Organism UniProt Comment Textmining
Treponema pectinovorum Q7X0P2 ATCC 33768
-

Purification (Commentary)

Purification (Comment) Organism
6*His-tagged recombinant enzyme Treponema pectinovorum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
esterified pectin 94% esterified pectin. 97% of the activity with polygalacturonic acid Treponema pectinovorum ?
-
?
pectin 80% of the activity with polygalacturonic acid Treponema pectinovorum ?
-
?
polygalacturonic acid
-
Treponema pectinovorum ?
-
?

Synonyms

Synonyms Comment Organism
PelA
-
Treponema pectinovorum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Treponema pectinovorum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 50 37°C: about 85% of maximal activity, 50°C: about 75% of maximal activity Treponema pectinovorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Treponema pectinovorum

pH Range

pH Minimum pH Maximum Comment Organism
8 10 pH 8.0: about 50% of maximal activity, pH 10.0: about 40% of maximal activity Treponema pectinovorum