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Literature summary for 4.2.2.17 extracted from
- Identification of a novel DFA I-producing inulin fructotransferase from Streptomyces davawensis (2016), Int. J. Biol. Macromol., 92, 723-730 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Streptomyces davaonensis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| homology modelling and molecular docking. Residues D183 and E194 are potential catalytic residues | Streptomyces davaonensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D183A | complete loss of activity | Streptomyces davaonensis |
| D183N | complete loss of activity | Streptomyces davaonensis |
| E194A | complete loss of activity | Streptomyces davaonensis |
| E194N | complete loss of activity | Streptomyces davaonensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.89 | - |
inulin | pH 5.5, 40°C | Streptomyces davaonensis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12500 | - |
gel fitration | Streptomyces davaonensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces davaonensis | - |
- |
- |
| Streptomyces davaonensis SK39.001 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fructofuranosyl nystose | - |
Streptomyces davaonensis | di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose | - |
? | |
| fructofuranosyl nystose | - |
Streptomyces davaonensis SK39.001 | di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose | - |
? | |
| inulin | - |
Streptomyces davaonensis | di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose + nystose + fructofuranosyl nystose | - |
? | |
| inulin | - |
Streptomyces davaonensis SK39.001 | di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose + nystose + fructofuranosyl nystose | - |
? | |
| additional information | no substrate: 1-kestose | Streptomyces davaonensis | ? | - |
? | |
| additional information | no substrate: 1-kestose | Streptomyces davaonensis SK39.001 | ? | - |
? | |
| nystose | - |
Streptomyces davaonensis | di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose | - |
? | |
| nystose | - |
Streptomyces davaonensis SK39.001 | di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 40000, SDS-PAGE, 3 * 40769, calculated from sequence | Streptomyces davaonensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Streptomyces davaonensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
2h, 40% residual activity | Streptomyces davaonensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Streptomyces davaonensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | more than 69% of maximum activity | Streptomyces davaonensis |
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Release 2023.1 (January 2023)
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