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Literature summary for 4.2.2.14 extracted from

  • Konno, N.; Ishida, T.; Igarashi, K.; Fushinobu, S.; Habu, N.; Samejima, M.; Isogai, A.
    Crystal structure of polysaccharide lyase family 20 endo-beta-1,4-glucuronan lyase from the filamentous fungus Trichoderma reesei (2009), FEBS Lett., 583, 1323-1326.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Trichoderma reesei

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 20% (w/v) PEG3350 and 0.2 M ammonium citrate buffer (pH 5.0) Trichoderma reesei

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ a calcium ion contributes to the enzyme stability Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Trichoderma reesei B6F143
-
-

Purification (Commentary)

Purification (Comment) Organism
phenyl-Toyopearl column chromatography and SuperQ-Toyopearl column chromatography Trichoderma reesei

Synonyms

Synonyms Comment Organism
endo-beta-(1->4)-glucuronan lyase
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Trichoderma reesei
endo-beta-1,4-glucuronan lyase
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Trichoderma reesei