Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.2.14 extracted from

  • Delattre, C.; Michaud, P.; Keller, C.; Elboutachfaiti, R.; Beven, L.; Courtois, B.; Courtois, J.
    Purification and characterization of a novel glucuronan lyase from Trichoderma sp. GL2 (2006), Appl. Microbiol. Biotechnol., 70, 437-443.
    View publication on PubMed
Search for more literature for 4.2.2.14

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 81% inhibition Trichoderma sp.
Fe2+ 1 mM, 19% inhibition Trichoderma sp.
Ni2+ 1 mM, 60% inhibition Trichoderma sp.
Rb+ 1 mM, 10% inhibition Trichoderma sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Trichoderma sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, enhances activity 4.56fold Trichoderma sp.
Co2+ 1 mM, enhances activity 1.5fold Trichoderma sp.
Li+ 1 mM, enhances activity 4.4fold Trichoderma sp.
Mg2+ 1 mM, enhances activity 2.5fold Trichoderma sp.
Mn2+ 1 mM, enhances activity 2.2fold Trichoderma sp.
additional information enzyme appears not to be really affected by ionic strength variations. The optimum is 300 mM concentration of potassium acetate buffer Trichoderma sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 x * 27000, SDS-PAGE Trichoderma sp.

Organism

Organism UniProt Comment Textmining
Trichoderma sp.
-
 GL2
-
Trichoderma sp. GL2
-
 GL2
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trichoderma sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
162
-
-
 Trichoderma sp.

Subunits

Subunits Comment Organism
? x * 27000, SDS-PAGE Trichoderma sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Trichoderma sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 1 h, stable below Trichoderma sp.
40
-
 1 h, 90% loss of activity Trichoderma sp.
60
-
 1 h, complete inactivation Trichoderma sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Trichoderma sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 30 min, stable Trichoderma sp.

pI Value

Organism Comment pI Value Maximum pI Value
Trichoderma sp. isoelectric focusing
-
 6.95