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Literature summary for 4.2.2.13 extracted from

  • Yoshinaga, K.; Fujisue, M.; Abe, J.; Hanashiro, I.; Takeda, Y.; Muroya, K.; Hizukuri, S.
    Characterization of exo-(1,4)-alpha glucan lyase from red alga Gracilaria chorda. Activation, inactivation and the kinetic properties of the enzyme (1999), Biochim. Biophys. Acta, 1472, 447-454.
    View publication on PubMed
Search for more literature for 4.2.2.13

General Stability

General Stability Organism
1 mM NaCl or 10 mM calcium acetate stabilize against thermal inactivation at 50°C Gracilariopsis chorda

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride
-
 Gracilariopsis chorda
2,4,6-trinitrobenzene-sulfonyl acid
-
 Gracilariopsis chorda
Cu2+ 10 mM, complete inactivation Gracilariopsis chorda
Hg2+ 1 mM, complete inactivation Gracilariopsis chorda
additional information no effect with 1 mM Mg2+, Zn2+, Fe2+ or Co2+ Gracilariopsis chorda
N-bromosuccinimide
-
 Gracilariopsis chorda

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 amylopectin pH 5.0, 35°C, barley amylopectin or potato amylopectin Gracilariopsis chorda
0.03
-
 glycogen pH 5.0, 35°C, rabbit liver glycogen Gracilariopsis chorda
0.1
-
 maltotetraose pH 5.0, 35°C Gracilariopsis chorda
0.1
-
 maltopentaose pH 5.0, 35°C Gracilariopsis chorda
0.1
-
 maltohexaose pH 5.0, 35°C Gracilariopsis chorda
0.1
-
 maltoheptaose pH 5.0, 35°C Gracilariopsis chorda
0.4
-
 maltotriose pH 5.0, 35°C Gracilariopsis chorda
2.3
-
 maltose pH 5.0, 35°C Gracilariopsis chorda

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1.3fold activation Gracilariopsis chorda

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 x * 100000, SDS-PAGE Gracilariopsis chorda

Organism

Organism UniProt Comment Textmining
Gracilariopsis chorda
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gracilariopsis chorda

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin barley amylopectin and potato amylopectin Gracilariopsis chorda ?
-
 ?
Glycogen rabbit liver glycogen Gracilariopsis chorda ?
-
 ?
Maltoheptaose
-
 Gracilariopsis chorda ?
-
 ?
Maltohexaose
-
 Gracilariopsis chorda ?
-
 ?
maltopentaose
-
 Gracilariopsis chorda ?
-
 ?
maltose
-
 Gracilariopsis chorda D-glucose + 1,5-anhydro-D-fructose
-
 ?
Maltotetraose
-
 Gracilariopsis chorda ?
-
 ?
maltotriose
-
 Gracilariopsis chorda ?
-
 ?

Subunits

Subunits Comment Organism
? x * 100000, SDS-PAGE Gracilariopsis chorda

Synonyms

Synonyms Comment Organism
GLase
-
 Gracilariopsis chorda

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 optimal activity without addition of any salt Gracilariopsis chorda
50
-
 optimal activity in presence of 1 mM calcium chloride Gracilariopsis chorda

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 55 35°C: about 50% of maximal activity, 55°C: about 80% of maximal activity, optimal activity in presence of 1 mM calcium chloride Gracilariopsis chorda
35 50 35°C: about 70% of maximal activity, 50°C: about 70% of maximal activity, activity without addition of any salt Gracilariopsis chorda

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 5.0, 20 min, 77% loss of activity without addition of any salt, 49% loss of activity in presence of 1 mM NaCl, 41% loss of activity in presence of 10 mM calcium acetate Gracilariopsis chorda