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Literature summary for 4.2.2.12 extracted from

  • Hashimoto, W.; Miki, H.; Tsuchiya, N.; Nankai, H.; Murata, K.
    Xanthan lyase of Bacillus sp. strain GL1 liberates pyruvylated mannose from xanthan side chains (1998), Appl. Environ. Microbiol., 64, 3765-3768.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 1 mM, slight stimulation Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, 30% loss of activity Bacillus sp. (in: Bacteria)
HgCl2 1 mM, 95% loss of activity Bacillus sp. (in: Bacteria)
iodoacetamide 1 mM, 70% loss of activity Bacillus sp. (in: Bacteria)
NaCl 150 mM, 70% loss of activity Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble excreted to medium Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM, slight stimulation Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
gel filtration Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
enzyme is induced by xanthan
-
Bacillus sp. (in: Bacteria) GL1
-
enzyme is induced by xanthan
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.28
-
pH 7.0 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvylated xanthan best substrate Bacillus sp. (in: Bacteria) pyruvylate mannose + oligosaccharides
-
?
pyruvylated xanthan best substrate Bacillus sp. (in: Bacteria) GL1 pyruvylate mannose + oligosaccharides
-
?
xanthan
-
Bacillus sp. (in: Bacteria) oligosaccharides
-
?
xanthan
-
Bacillus sp. (in: Bacteria) GL1 oligosaccharides
-
?

Subunits

Subunits Comment Organism
monomer 1 * 75000, SDS-PAGE   Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
pH 7.0, 10 min, loss of 60% of activity Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 at 4°C Bacillus sp. (in: Bacteria)