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Literature summary for 4.2.2.11 extracted from

  • Yagi, H.; Fujise, A.; Itabashi, N.; Ohshiro, T.
    Characterization of a novel endo-type alginate lyase derived from Shewanella sp. YH1 (2018), J. Biochem., 163, 341-350 .
    View publication on PubMed
Search for more literature for 4.2.2.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Shewanella sp. YH1

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 1 mM, 59% residual activity Shewanella sp. YH1
Ba2+ 1 mM, 13% residual activity Shewanella sp. YH1
Ca2+ 1 mM, 36% residual activity Shewanella sp. YH1
Co2+ 1 mM, 12% residual activity Shewanella sp. YH1
Cu2+ 1 mM, 8% residual activity Shewanella sp. YH1
EDTA 1 mM, 8% residual activity Shewanella sp. YH1
Fe3+ 1 mM, 60% residual activity Shewanella sp. YH1
Mn3+ 1 mM, 8% residual activity Shewanella sp. YH1
additional information enzymatic activity is about two times higher in phosphate buffer than in Tris buffer Shewanella sp. YH1
N-bromosuccinimide 1 mM, no residual activity Shewanella sp. YH1
Ni2+ 1 mM, 20% residual activity Shewanella sp. YH1

Organism

Organism UniProt Comment Textmining
Shewanella sp. YH1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native and recombinant protein Shewanella sp. YH1

Renatured (Commentary)

Renatured (Comment) Organism
enzyme is not thermotolerant, but can refold to its active form following an almost complete denaturation at approximately 60°C Shewanella sp. YH1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate
-
 Shewanella sp. YH1 unsaturated algino-oligosaccharides
-
 ?
alginate about 50% of the activity with poly(alpha-(1,4)-L-guluronate) Shewanella sp. YH1 unsaturated algino-oligosaccharides
-
 ?
poly(alpha-(1,4)-L-guluronate)
-
 Shewanella sp. YH1 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?
poly(beta-(1,4)-D-mannuronate) reaction of EC 4.2.2.3, about 20% of the activity with poly(alpha-(1,4)-L-guluronate) Shewanella sp. YH1 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
 ?
poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate) about 30% of the activity with poly(alpha-(1,4)-L-guluronate) Shewanella sp. YH1 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) + 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE, x * 36376, calculated from sequence Shewanella sp. YH1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Shewanella sp. YH1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Shewanella sp. YH1

pH Range

pH Minimum pH Maximum Comment Organism
7 9 at least 70% of maximum activity Shewanella sp. YH1

pI Value

Organism Comment pI Value Maximum pI Value
Shewanella sp. YH1 calculated from sequence
-
 6