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Literature summary for 4.2.2.11 extracted from
- Purification and characterization of an alkaliphilic alginate lyase AlgMytC from Saccharophagus sp. Myt-1 (2013), J. Microbiol. Biotechnol., 23, 872-877.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | slightly activating at 0.001%, slightly inhibitory at 0.01% | Saccharophagus sp. Myt-1 |  |
| Tween 20 | slightly activating at 0.001-0.01% | Saccharophagus sp. Myt-1 | |
| Tween 40 | slightly activating at 0.001-0.01% | Saccharophagus sp. Myt-1 | |
| Tween 80 | slightly activating at 0.001-0.01% | Saccharophagus sp. Myt-1 | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene algMytC, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) partly in inclusion bodies | Saccharophagus sp. Myt-1 |
General Stability
| General Stability | Organism |
|---|---|
| activity of AlgMytC is stable in the presence of various detergents | Saccharophagus sp. Myt-1 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibition at 1-5 mM | Saccharophagus sp. Myt-1 | |
| EDTA | - |
Saccharophagus sp. Myt-1 | |
| Mg2+ | inhibition at 1-5 mM | Saccharophagus sp. Myt-1 | |
| additional information | activity of AlgMytC is stable in the presence of various detergents | Saccharophagus sp. Myt-1 | |
| Triton X-100 | slightly activating at 0.001%, slightly inhibitory at 0.01% | Saccharophagus sp. Myt-1 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | a metal ion is required. No or poor effects by SDS at 0.001-0.01%, and DTT, Na+ and K+ at 1-5 mM | Saccharophagus sp. Myt-1 |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37573 | - |
x * 37573, sequence calculation, x * 43000, recombinant His-tagged enzyme, SDS-PAGE | Saccharophagus sp. Myt-1 |
| 43000 | - |
x * 37573, sequence calculation, x * 43000, recombinant His-tagged enzyme, SDS-PAGE | Saccharophagus sp. Myt-1 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharophagus sp. Myt-1 | L8B3Z6 | gene algMytC | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant soluble and renatured His-tagged enzyme from Escherichia coli strain BL21(DE3) | Saccharophagus sp. Myt-1 |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| solubilization of enzyme from inclusion bodies by 8 M urea in Tris-HCl, pH 8.0 | Saccharophagus sp. Myt-1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alginate | AlgMytC is predicted to preferably degrade guluronate-blocks because of the presence of the QIH motif in the conserved signature of the amino acid sequence | Saccharophagus sp. Myt-1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37573, sequence calculation, x * 43000, recombinant His-tagged enzyme, SDS-PAGE | Saccharophagus sp. Myt-1 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AlgMytC | - |
Saccharophagus sp. Myt-1 |
| alkaliphilic alginate lyase | - |
Saccharophagus sp. Myt-1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Saccharophagus sp. Myt-1 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 45 | 30% of maximal activity at 15°C and 45°C | Saccharophagus sp. Myt-1 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 35 | purified recombinant enzyme, pH 9.0, completely stable at 25°C for 24 h and at 35°C for 1 h, but loss of 60% activity after 24 h at 35°C | Saccharophagus sp. Myt-1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Saccharophagus sp. Myt-1 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | 90% of maximal activity at pH 8.5 and pH 10.0, 30% at pH 7.5, inactive below, profile overview | Saccharophagus sp. Myt-1 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | purified recombinant enzyme, 25°C, 24 h, over 70% activity remaining | Saccharophagus sp. Myt-1 |
| 8.5 | 10 | purified recombinant enzyme, 25°C, 24 h, over 90% activity remaining | Saccharophagus sp. Myt-1 |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharophagus sp. Myt-1 | sequence calculation | - |
6.6 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme contains the conserved amino acid sequences RTELREM, QIH, and YFKAGVYNQ of the polysaccharide lyase family 7 | Saccharophagus sp. Myt-1 |
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