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Literature summary for 4.2.2.11 extracted from

  • Nakagawa, A.; Ozaki, T.; Chubachi, K.; Hosoyama, T.; Okubo, T.; Iyobe, S.; Suzuki, T.
    An effective method for isolating alginate lyase-producing Bacillus sp. ATB-1015 strain and purification and characterization of the lyase (1998), J. Appl. Microbiol., 84, 328-335.
    View publication on PubMed
Search for more literature for 4.2.2.11

Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition at 1 mM concentration, almost completely recovered activity by the addition of 2 mM CaCl2 or partially recovered by the addition of CoCl2 or MnCl2, suggesting that the enzyme conformation is bivalent-cation dependent Bacillus sp. (in: Bacteria)
Guanidinium chloride concentrations above 3 M cause significant loss of activity Bacillus sp. (in: Bacteria)
additional information 10 mM SDS, protein denaturants or 1 mM urea do not affect enzyme activity Bacillus sp. (in: Bacteria)
Urea 36% inhibition in the presence of 8 M urea Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 2 mM, 66% inhibition Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 Sephacryl S-200 HR column chromatography Bacillus sp. (in: Bacteria)
41000
-
 1 * 41000, SDS-PAGE Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly-alpha-L-guluronic acid Bacillus sp. (in: Bacteria)
-
 unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate
-
 ?
poly-alpha-L-guluronic acid Bacillus sp. (in: Bacteria) ATB-1015
-
 unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 ATB-1015 strain
-
Bacillus sp. (in: Bacteria) ATB-1015
-
 ATB-1015 strain
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, gel filtration and anion-exchange chromatography Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly-alpha-L-guluronic acid
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
poly-alpha-L-guluronic acid
-
 Bacillus sp. (in: Bacteria) ATB-1015 ?
-
 ?
poly-alpha-L-guluronic acid
-
 Bacillus sp. (in: Bacteria) unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate
-
 ?
poly-alpha-L-guluronic acid
-
 Bacillus sp. (in: Bacteria) ATB-1015 unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate
-
 ?
poly-beta1,4-D-mannuronan seems to have slight activity on poly-mannuronan Bacillus sp. (in: Bacteria) ?
-
 ?
poly-beta1,4-D-mannuronan seems to have slight activity on poly-mannuronan Bacillus sp. (in: Bacteria) ATB-1015 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 41000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 50% of the activity remains after treatment at this temperature for 30 min Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
7 8 about 70% activity retained at pH 9.0 Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 relatively stable in alkaline pH and unstable in a range outside acidic pH 5.0 Bacillus sp. (in: Bacteria)