Literature summary for 4.2.2.10 extracted from

Hadj Sassi, A.; Trigui-Lahiani, H.; Abdeljalil, S.; Gargouri, A.
Enhancement of solubility, purification and inclusion-bodies-refolding of an active pectin lyase from Penicillium occitanis expressed in Escherichia coli (2017), Int. J. Biol. Macromol., 95, 256-262 .

Search for more literature for 4.2.2.10

Application

Application	Comment	Organism
synthesis	maximal production of functional Pnl1 is obtained after induction by 0.4 mM isopropyl beta-D-thiogalactoside at 30°C and 150 rpm for 16 h. The use of Origami host strain significantly improves the yield of the soluble active form of the enzyme	Penicillium sp. 'occitanis'

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Penicillium sp. 'occitanis'

Organism

Organism	UniProt	Comment	Textmining
Penicillium sp. 'occitanis'	Q0PH38	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein, single step purification using His-Trap affinity column chromatography	Penicillium sp. 'occitanis'

Subunits

Subunits	Comment	Organism
?	x * 42000, SDS-PAGE	Penicillium sp. 'occitanis'

Synonyms

Synonyms	Comment	Organism
Pnl1	-	Penicillium sp. 'occitanis'

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)