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Literature summary for 4.2.2.10 extracted from
- Purification and characterization of alkaline pectin lyase from a newly isolated Bacillus clausii and its application in elicitation of plant disease resistance (2012), Appl. Biochem. Biotechnol., 167, 2241-2256.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | purified enzyme is able to elicit disease resistance in cucumber seedlings | Alkalihalobacillus clausii |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Alkalihalobacillus clausii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1 mM, no residual activity | Alkalihalobacillus clausii |  |
| Mn2+ | 1 mM, no residual activity | Alkalihalobacillus clausii | |
| Zn2+ | 1 mM, 44.6% residual activity | Alkalihalobacillus clausii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
apple pectin | Km value is 0.87 mg/ml, pH 10, 60°C | Alkalihalobacillus clausii |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 410% iof initial activity | Alkalihalobacillus clausii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
1 * 35000, SDS-PAGE | Alkalihalobacillus clausii |
| 35880 | - |
MALDI-TOF/TOF | Alkalihalobacillus clausii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alkalihalobacillus clausii | AY825251 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification from culture fluid | Alkalihalobacillus clausii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture fluid | - |
Alkalihalobacillus clausii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 297 | - |
pH 10, 60°C | Alkalihalobacillus clausii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| apple pectin | - |
Alkalihalobacillus clausii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 35000, SDS-PAGE | Alkalihalobacillus clausii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Alkalihalobacillus clausii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | 65 | more than 60% of maximum activity within | Alkalihalobacillus clausii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
30 min, 68% residual activity | Alkalihalobacillus clausii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Alkalihalobacillus clausii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 10.5 | more than 50% of maximum activity within | Alkalihalobacillus clausii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 11 | - |
Alkalihalobacillus clausii |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Alkalihalobacillus clausii | use of sugar beet pulp as carbon source an inducer. Enzyme secretion occurs over the temperature range of 30°C to 45°C, and the optimum temperature is 40°C | up |
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