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Literature summary for 4.2.2.10 extracted from

  • Schlemmer, A.F.; Ware, C.F.; Keen, N.T.
    Purification and characterization of a pectin lyase produced by Pseudomonas fluorescens W51 (1987), J. Bacteriol., 169, 4493-4498.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.2.10

General Stability

General Stability Organism
20% loss of activity after overnight dialysis against 10 mM potassium phosphate, pH 7.5. 50% loss of activity after overnight dialysis against 10 mM Tris-HCl, pH 7.5. Complete loss of activity after dialysis against water Pseudomonas fluorescens
freezing and thawing causes 20% inactivation Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km for citrus pectin: 3.2 mg/ml Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 x * 32000, SDS-PAGE Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas fluorescens constitutive enzyme, probably involved in the soft rot disease caused by Pseudomonas fluorescens ?
-
 ?
additional information Pseudomonas fluorescens W51 constitutive enzyme, probably involved in the soft rot disease caused by Pseudomonas fluorescens ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
 W51
-
Pseudomonas fluorescens W51
-
 W51
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information constitutive enzyme, probably involved in the soft rot disease caused by Pseudomonas fluorescens Pseudomonas fluorescens ?
-
 ?
additional information constitutive enzyme, probably involved in the soft rot disease caused by Pseudomonas fluorescens Pseudomonas fluorescens W51 ?
-
 ?
pectin
-
 Pseudomonas fluorescens ?
-
 ?
pectin
-
 Pseudomonas fluorescens W51 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Pseudomonas fluorescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 room temperature, 5 min, 30% loss of activity Pseudomonas fluorescens
36
-
 10 min, 30% loss of activity Pseudomonas fluorescens
42
-
 5 min, 30% loss of activity Pseudomonas fluorescens
68
-
 pH 7.8, 5 min, 97% loss of activity Pseudomonas fluorescens

pH Range

pH Minimum pH Maximum Comment Organism
8 8.5 citrus pectin, Tris-HCl buffer Pseudomonas fluorescens