Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Hydroxy-5-nitrobenzylbromide | 2.5 mM, 87.4% inhibition | Neobacillus niacini | |
dithiothreitol | 5 mM, 30% inhibition | Neobacillus niacini | |
iodoacetate | 5 mM, slight inhibition | Neobacillus niacini | |
N-bromosuccinimide | 5 mM, slight inhibition | Neobacillus niacini | |
N-ethylmaleimide | 5 mM, slight inhibition | Neobacillus niacini | |
p-chloromercuribenzoate | 5 mM, 30% inhibition | Neobacillus niacini |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Neobacillus niacini | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | optimum concentrations are 5, 50, and 100 mM calcium ions for degradation of hyaluronan, chondroitin sulfate C, and chondroitin sulfate A, respectively | Neobacillus niacini | |
Mn2+ | 82% of the activity with Ca2+ | Neobacillus niacini | |
additional information | less than 20% of maximum activity in absence of metal ions | Neobacillus niacini | |
Sr2+ | 78.6% of the activity with Ca2+ | Neobacillus niacini |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neobacillus niacini | X5I0R2 | - |
- |
Neobacillus niacini JAM F8 | X5I0R2 | - |
- |
Purification (Comment) | Organism |
---|---|
from a culture broth | Neobacillus niacini |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
136.7 | - |
substrate hyaluronan, pH 6.0, 37°C | Neobacillus niacini |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chondroitin sulfate A | 44% of the activity with hyaluronan | Neobacillus niacini | ? | - |
? | |
chondroitin sulfate A | 44% of the activity with hyaluronan | Neobacillus niacini JAM F8 | ? | - |
? | |
chondroitin sulfate C | 30% of the activity with hyaluronan | Neobacillus niacini | 2-acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-D-galactose | - |
? | |
chondroitin sulfate C | 30% of the activity with hyaluronan | Neobacillus niacini JAM F8 | 2-acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-D-galactose | - |
? | |
hyaluronan | enzyme displays endo-type degratation. The viscosity of hyaluronan decreases to 37% of the original one after 3-min incubation | Neobacillus niacini | 2-acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-D-glucose | - |
? | |
hyaluronan | enzyme displays endo-type degratation. The viscosity of hyaluronan decreases to 37% of the original one after 3-min incubation | Neobacillus niacini JAM F8 | 2-acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-D-glucose | - |
? | |
additional information | chondroitin, 2.3% of the activity with hyaluronan | Neobacillus niacini | ? | - |
? | |
additional information | chondroitin, 2.3% of the activity with hyaluronan | Neobacillus niacini JAM F8 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 120000, SDS-PAGE | Neobacillus niacini |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Neobacillus niacini |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10% of maximum activity | Neobacillus niacini |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
15 min, absence of Ca2+, 30% residual lactivity | Neobacillus niacini |
45 | - |
15 min, stable in presence of Ca2+ | Neobacillus niacini |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Neobacillus niacini |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | stable in presence of Ca2+ | Neobacillus niacini |
6 | 10 | stable in absence of Ca2+ | Neobacillus niacini |