Literature summary for 4.2.2.1 extracted from

Sun, X.; Wang, Z.; Bi, Y.; Wang, Y.; Liu, H.
Genetic and functional characterization of the hyaluronate lyase HylB and the beta-N-acetylglucosaminidase HylZ in Streptococcus zooepidemicus (2015), Curr. Microbiol., 70, 35-42 .

Search for more literature for 4.2.2.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Streptococcus equi subsp. zooepidemicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00057	-	hyaluronan	pH 6.0, 37°C	Streptococcus equi subsp. zooepidemicus

Organism

Organism	UniProt	Comment	Textmining
Streptococcus equi subsp. zooepidemicus	W6E7E9	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hyaluronan	-	Streptococcus equi subsp. zooepidemicus	3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine	-	?

Synonyms

Synonyms	Comment	Organism
hylB	-	Streptococcus equi subsp. zooepidemicus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Streptococcus equi subsp. zooepidemicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Streptococcus equi subsp. zooepidemicus

Expression

Organism	Comment	Expression
Streptococcus equi subsp. zooepidemicus	expression level of hylB is growth-phase dependent	additional information

General Information

General Information	Comment	Organism
physiological function	loss of hylB or/and constitutively expressed hylZ has no significant effects on cell growth, lactic acid, and hyaluronan yields. The HylB mutant strain produces hyaluronan with an 18 % increase in molecular weight	Streptococcus equi subsp. zooepidemicus

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Release 2023.1 (January 2023)