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Literature summary for 4.2.2.1 extracted from

  • Zhu, C.; Zhang, J.; Li, L.; Zhang, J.; Jiang, Y.; Shen, Z.; Guan, H.; Jiang, X.
    Purification and characterization of hyaluronate lyase from Arthrobacter globiformis A152 (2017), Appl. Biochem. Biotechnol., 182, 216-228 .
    View publication on PubMed
Search for more literature for 4.2.2.1

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 5 mM, 75% residual activity Arthrobacter globiformis
Cu2+ 50 mM, no residual activity Arthrobacter globiformis
EDTA 5 mM, 84% residual activity Arthrobacter globiformis
hyaluronate substrate inhibition above 7.5 mg/ml Arthrobacter globiformis
Li+ 50 mM, 68% residual activity Arthrobacter globiformis
Mg2+ 50 mM, 68% residual activity Arthrobacter globiformis
SDS 5 mM, 4.6% residual activity Arthrobacter globiformis
Tween 80 5 mM, 84% residual activity Arthrobacter globiformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 hyaluronate Km value 0.11 mg/ml, pH 6.5, 37°C Arthrobacter globiformis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 50 mM, 111% of initial activity Arthrobacter globiformis

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis
-
-
-
Arthrobacter globiformis A152
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification from the supernatant by ammonium sulfate fractionation, Q Sepharose Fast Flow, and Sephadex G-100 chromatography Arthrobacter globiformis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
297.2
-
 pH 6.5, 37°C Arthrobacter globiformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin sulfate A 24.7% of the activity with hyaluronate Arthrobacter globiformis ?
-
 ?
chondroitin sulfate A 24.7% of the activity with hyaluronate Arthrobacter globiformis A152 ?
-
 ?
hyaluronate
-
 Arthrobacter globiformis 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
-
 ?
hyaluronate
-
 Arthrobacter globiformis A152 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
-
 ?
additional information very poor substrate: chitosan. No substrates: heparin sodium, sodium alginate, carrageenan, pectin, soluble starch, xanthan gum Arthrobacter globiformis ?
-
 ?
additional information very poor substrate: chitosan. No substrates: heparin sodium, sodium alginate, carrageenan, pectin, soluble starch, xanthan gum Arthrobacter globiformis A152 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 73700, SDS-PAGE Arthrobacter globiformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
-
 Arthrobacter globiformis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 at least 47% of maximum activity Arthrobacter globiformis
37
-
 more than 76% of maximum activity Arthrobacter globiformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 37 120 min, more than 84% residual activity Arthrobacter globiformis
42
-
 60 min, 43% residual activity Arthrobacter globiformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Arthrobacter globiformis

pH Range

pH Minimum pH Maximum Comment Organism
5 9
-
 Arthrobacter globiformis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 1 h, 37°C, 81% residual activity Arthrobacter globiformis