Literature summary for 4.2.2.1 extracted from

Lindsay, A.M.; Zhang, M.; Mitchell, Z.; Holden, M.T.; Waller, A.S.; Sutcliffe, I.C.; Black, G.W.
The Streptococcus equi prophage-encoded protein SEQ2045 is a hyaluronan-specific hyaluronate lyase that is produced during equine infection (2009), Microbiology, 155, 443-449.

Search for more literature for 4.2.2.1

Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged version expressed in Escherichia coli BL21(DE3)	Streptococcus equi subsp. equi 4047

Protein Variants

Protein Variants	Comment	Organism
D171A	catalytic mutant, 8% activity	Streptococcus equi subsp. equi 4047
Y183A	catalytic mutant, 13% activity	Streptococcus equi subsp. equi 4047

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	not inhibited by ascorbic acid at concentration up to 20 mM	Streptococcus equi subsp. equi 4047

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.71	-	hyaluronic acid	concentration expressed as the concentration of its disaccharide unit (equivalent to 0.29 mg/ml)	Streptococcus equi subsp. equi 4047

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	no requirement for any specific divalent ion tested	Streptococcus equi subsp. equi 4047

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
42000	-	x * 42000, SDS-PAGE	Streptococcus equi subsp. equi 4047

Organism

Organism	UniProt	Comment	Textmining
Streptococcus equi subsp. equi 4047	C0M9W4	prophage encoded	-

Purification (Commentary)

Purification (Comment)	Organism
-	Streptococcus equi subsp. equi 4047

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hyaluronic acid	no activity against chondroitin 4-sulfate, chondroitin 6-sulfate and dermatan sulfate	Streptococcus equi subsp. equi 4047	?	end-products are 4,5-unsaturated hyalurono-hexasaccharide and 4,5-unsaturated hyalurono-octasaccharide, with small amounts of 4,5-unsaturated hyalurono-tetrasaccharide, 4,5-unsaturated hyalurono-decasaccharide and longer oligosaccharides, early time points shows that a wide range of 4,5-unsaturated oligosaccharides are produced, indicating an endo-acting mode of digestion	?

Subunits

Subunits	Comment	Organism
?	x * 42000, SDS-PAGE	Streptococcus equi subsp. equi 4047

Synonyms

Synonyms	Comment	Organism
hyaluronate lyase	-	Streptococcus equi subsp. equi 4047
protein SEQ2045	-	Streptococcus equi subsp. equi 4047

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	50% activity at 44°C	Streptococcus equi subsp. equi 4047

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
7.61	-	hyaluronic acid	-	Streptococcus equi subsp. equi 4047

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Streptococcus equi subsp. equi 4047

General Information

General Information	Comment	Organism
physiological function	produced during infection of horses	Streptococcus equi subsp. equi 4047

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)