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Literature summary for 4.2.2.1 extracted from
- Unusual structural features of the bacteriophage-associated hyaluronate lyase (hylp2) (2006), J. Biol. Chem., 281, 7143-7150.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Streptococcus pyogenes phage H10403 |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
recombinant enzyme, gel filtration | Streptococcus pyogenes phage H10403 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hyaluronan | Streptococcus pyogenes phage H10403 | absolute substrate specificity of the bacteriophage enzyme | hyaluronic acid oligomers | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus pyogenes phage H10403 | - |
gene hylp2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to homogeneity by ion exchange and nickel affinity chromatography, and gel filtration | Streptococcus pyogenes phage H10403 |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| [hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate | active site structure | Streptococcus pyogenes phage H10403 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hyaluronan | absolute substrate specificity of the bacteriophage enzyme | Streptococcus pyogenes phage H10403 | hyaluronic acid oligomers | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | three-dimensional structure analysis, unfolding process using 1-4 M guanidinium hydrochloride, effects of unfolding on the structure, transitions, kinetics, overview | Streptococcus pyogenes phage H10403 |
| trimer | 3 * 36000-36900, SDS-PAGE and ESI-MS | Streptococcus pyogenes phage H10403 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bacteriophage-associated hyaluronate lyase | - |
Streptococcus pyogenes phage H10403 |
| Hylp2 | - |
Streptococcus pyogenes phage H10403 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Streptococcus pyogenes phage H10403 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Streptococcus pyogenes phage H10403 |
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Release 2023.1 (January 2023)
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