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Literature summary for 4.2.2.1 extracted from

  • Kelly, S.J.; Taylor, K.B.; Li, S.; Jedrzejas, M.J.
    Kinetic properties of Streptococcus pneumoniae hyaluronate lyase (2001), Glycobiology, 11, 297-304.
    View publication on PubMed
Search for more literature for 4.2.2.1

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of wild-type and mutant enzymes in Escherichia coli Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic mechanism, kinetics, pH 6.0, 22°C Streptococcus pneumoniae
0.05
-
 hyaluronan recombinant mutant N349A, pH 6.0, 22°C Streptococcus pneumoniae
0.08
-
 hyaluronan hexasaccharide recombinant wild-type enzyme, pH 6.0, 22°C Streptococcus pneumoniae
0.12
-
 hyaluronan recombinant wild-type enzyme and mutant Y408F, pH 6.0, 22°C Streptococcus pneumoniae
0.17
-
 hyaluronan recombinant mutantR243V, pH 6.0, 22°C Streptococcus pneumoniae
0.99
-
 hyaluronan recombinant mutants H399A and N580A, pH 6.0, 22°C Streptococcus pneumoniae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane cell surface Streptococcus pneumoniae 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Escherichia coli Streptococcus pneumoniae

Reaction

Reaction Comment Organism Reaction ID
[hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate 5-step reaction mechanism, catalytic residues are Arg349, His399, Tyr40, residues responsible for substrate binding and translocation of the remaining substrate are Arg243and Asn580, enzyme and acitive site strucure Streptococcus pneumoniae
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hyaluronan progressive mode of action, degradation Streptococcus pneumoniae 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine unsaturated disaccharide unit ?
hyaluronan hexasaccharide progressive mode of action, degradation Streptococcus pneumoniae 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine unsaturated disaccharide unit ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Streptococcus pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Streptococcus pneumoniae