Cloned (Comment) | Organism |
---|---|
overexpression of fully functional truncated wild-type and mutant enzymes in Escherichia coli | Streptococcus pneumoniae |
Crystallization (Comment) | Organism |
---|---|
purified recombinant truncated wid-type enzyme and selenomethionine derivative, comprising residues Ala168-Ala893, in 3.5 M ammonium sulfate, 200 mM sodium cacodylate, pH 6.0, cryoprotection in 30% xylitol, heavy atom derivative X-ray diffraction structure determination and analysis at 1.56 A resolution | Streptococcus pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
H399A | site-directed mutagenesis, highly reduced activity compared to wild-type enzyme | Streptococcus pneumoniae |
N349A | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Streptococcus pneumoniae |
N580G | site-directed mutagenesis, slightly increased activity compared to wild-type enzyme | Streptococcus pneumoniae |
R243V | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Streptococcus pneumoniae |
Y408F | site-directed mutagenesis, inactive mutant | Streptococcus pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | binding modulates enzyme structure | Streptococcus pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate | catalytic mechanism, substrate binding, structure modeling, residues Tyr408, Asn349, and His399 are involved in catalysis and important | Streptococcus pneumoniae |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hyaluronan | - |
Streptococcus pneumoniae | 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | N-terminal alpha- and C-terminal beta-domain structure determination, determination of interface residues, overview | Streptococcus pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
bacterial spreading factor | - |
Streptococcus pneumoniae |
spnHL | - |
Streptococcus pneumoniae |