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Literature summary for 4.2.2.1 extracted from

  • Li, S.; Kelly, S.J.; Lamani, E.; Ferraroni, M.; Jedrzejas, M.J.
    Structural basis of hyaluronan degradation by Streptococcus pneumoniae hyaluronate lyase (2000), EMBO J., 19, 1228-1240.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of fully functional truncated wild-type and mutant enzymes in Escherichia coli Streptococcus pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant truncated wid-type enzyme and selenomethionine derivative, comprising residues Ala168-Ala893, in 3.5 M ammonium sulfate, 200 mM sodium cacodylate, pH 6.0, cryoprotection in 30% xylitol, heavy atom derivative X-ray diffraction structure determination and analysis at 1.56 A resolution Streptococcus pneumoniae

Protein Variants

Protein Variants Comment Organism
H399A site-directed mutagenesis, highly reduced activity compared to wild-type enzyme Streptococcus pneumoniae
N349A site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Streptococcus pneumoniae
N580G site-directed mutagenesis, slightly increased activity compared to wild-type enzyme Streptococcus pneumoniae
R243V site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus pneumoniae
Y408F site-directed mutagenesis, inactive mutant Streptococcus pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ binding modulates enzyme structure Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
-
-

Reaction

Reaction Comment Organism Reaction ID
[hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate catalytic mechanism, substrate binding, structure modeling, residues Tyr408, Asn349, and His399 are involved in catalysis and important Streptococcus pneumoniae

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hyaluronan
-
Streptococcus pneumoniae 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
-
?

Subunits

Subunits Comment Organism
More N-terminal alpha- and C-terminal beta-domain structure determination, determination of interface residues, overview Streptococcus pneumoniae

Synonyms

Synonyms Comment Organism
bacterial spreading factor
-
Streptococcus pneumoniae
spnHL
-
Streptococcus pneumoniae