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Literature summary for 4.2.2.1 extracted from

  • Baker, J.R.; Dong, S.; Pritchard, D.G.
    The hyaluronan lyase of Streptococcus pyogenes bacteriophage H4489A (2002), Biochem. J., 365, 317-322.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Streptococcus pyogenes phage H4489A

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular in the bacterial capsule Streptococcus pyogenes phage H4489A
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39500
-
 x * 39500, SDS-PAGE Streptococcus pyogenes phage H4489A

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hyaluronan Streptococcus pyogenes phage H4489A extracellular enzyme facilitates the invasion and spreading of the pathogenic bacterium in the host tissue by degrading the hosts' extracellular matrix component in connective tissue ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes phage H4489A
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially, recombinant enzyme from Escherichia coli Streptococcus pyogenes phage H4489A

Reaction

Reaction Comment Organism Reaction ID
[hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate also acts on chondroitin, the product is more systematically known as 3-(4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid)-2-acetamido-2-deoxy-D-glucose Streptococcus pyogenes phage H4489A
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Streptococcus pyogenes phage H4489A

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hyaluronan extracellular enzyme facilitates the invasion and spreading of the pathogenic bacterium in the host tissue by degrading the hosts' extracellular matrix component in connective tissue Streptococcus pyogenes phage H4489A ?
-
 ?
hyaluronan + H2O specific for, degradation Streptococcus pyogenes phage H4489A oligosaccharides
-
 ?
additional information substrate specificity, no activity with chonroitin, dermatan sulfate, keratan sulfate, heparin, chondroitin 4-sulfate and chondroitin 6-sulfate Streptococcus pyogenes phage H4489A ?
-
 ?

Subunits

Subunits Comment Organism
? x * 39500, SDS-PAGE Streptococcus pyogenes phage H4489A

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Streptococcus pyogenes phage H4489A

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.9
-
 hyaluronan recombinant enzyme, pH 6.0, 30°C Streptococcus pyogenes phage H4489A

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Streptococcus pyogenes phage H4489A

pI Value

Organism Comment pI Value Maximum pI Value
Streptococcus pyogenes phage H4489A isoelectric focusing 4.7 4.6