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Literature summary for 4.2.1.98 extracted from
- Purification and properties of 16alpha-hydroxyprogesterone dehydroxylase from Eubacterium sp. Strain 144 (1985), Biochim. Biophys. Acta, 837, 103-110.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| SDS | 0.1%, stimulates | Eubacterium sp. |  |
| SDS | 0.05%, about 7fold stimulation | Eubacterium sp. | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 16-Ketoprogesterone | competitive with respect to 16alpha-hydroxypregnenolone and 16alpha-hydroxyprogesterone | Eubacterium sp. | |
| 16alpha-hydroxypregnenolone | substrate inhibition at high concentrations | Eubacterium sp. | |
| cetyltrimethylammonium bromide | no inhibition by NaBr | Eubacterium sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
16alpha-hydroxypregnenolone | - |
Eubacterium sp. | |
| 0.52 | - |
16alpha-hydroxyprogesterone | - |
Eubacterium sp. | |
| 0.52 | - |
16alpha-hydroxyprogesterone | pH 6.8 | Eubacterium sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42400 | - |
4 * 42400, the enzyme is functional as tetramer and as octamer, SDS-PAGE | Eubacterium sp. |
| 42400 | - |
8 * 42400, the enzyme is functional as tetramer and as octamer, SDS-PAGE | Eubacterium sp. |
| 42400 | - |
x * 42400, SDS-PAGE | Eubacterium sp. |
| 181000 | - |
tetrameric form, gel filtration | Eubacterium sp. |
| 181000 | - |
active in two molecular weight forms, gel filtration | Eubacterium sp. |
| 326000 | - |
octameric form, gel filtration | Eubacterium sp. |
| 326000 | - |
active in two molecular weight forms, gel filtration | Eubacterium sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Eubacterium sp. | - |
- |
- |
| Eubacterium sp. 144 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Eubacterium sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.7 | - |
- |
Eubacterium sp. |
| 0.7094 | - |
- |
Eubacterium sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 16alpha-Hydroxypregnenolone | - |
Eubacterium sp. | ? | - |
? | |
| 16alpha-Hydroxypregnenolone | - |
Eubacterium sp. 144 | ? | - |
? | |
| 16alpha-hydroxypregnenolone | - |
Eubacterium sp. | 16-dehydropregnenolone + H2O | - |
? | |
| 16alpha-hydroxypregnenolone | - |
Eubacterium sp. 144 | 16-dehydropregnenolone + H2O | - |
? | |
| 16alpha-Hydroxyprogesterone | - |
Eubacterium sp. | 16-Dehydroprogesterone | - |
? | |
| 16alpha-Hydroxyprogesterone | - |
Eubacterium sp. 144 | 16-Dehydroprogesterone | - |
? | |
| 16alpha-hydroxyprogesterone | - |
Eubacterium sp. | 16dehydroprogesterone + H2O | - |
? | |
| 16alpha-hydroxyprogesterone | - |
Eubacterium sp. 144 | 16dehydroprogesterone + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42400, SDS-PAGE | Eubacterium sp. |
| octamer | 8 * 42400, the enzyme is functional as tetramer and as octamer, SDS-PAGE | Eubacterium sp. |
| tetramer | 4 * 42400, the enzyme is functional as tetramer and as octamer, SDS-PAGE | Eubacterium sp. |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
16-Ketoprogesterone | pH 6.8, reaction with 16alpha-hydroxyprogesterone | Eubacterium sp. | |
| 0.18 | - |
16-Ketoprogesterone | pH 6.8, reaction with 16alpha-hydroxypregnenolone | Eubacterium sp. | |
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