Literature summary for 4.2.1.98 extracted from

  • Glass, T.L.; Lamppa, R.S.; Hylemon, P.B.
    Characterization of 16alpha-hydroxyprohgesterone dehydroxylase in cell extracts of the intestinal anaerobic bacterium Eubacterium sp. 144 (1984), Biochim. Biophys. Acta, 792, 59-64.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
methanol active at 15-30% v/v methanol with an optimal concentration of 25% Eubacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
butanol
-
Eubacterium sp.
HgCl2 0.1 mM, 87% inhibition Eubacterium sp.
iodoacetic acid slight Eubacterium sp.
Isopropanol
-
Eubacterium sp.
n-butanol
-
Eubacterium sp.
NEM slight Eubacterium sp.
n-Propanol
-
Eubacterium sp.
PCMB 0.5 mM, 50% inhibition Eubacterium sp.
Propanol
-
Eubacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.56
-
16alpha-hydroxyprogesterone
-
Eubacterium sp.
0.56
-
16alpha-hydroxyprogesterone pH 6.8, 37°C Eubacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
285000
-
gel filtration Eubacterium sp.

Organic Solvent Stability

Organic Solvent Comment Organism
Methanol high tolerance to Eubacterium sp.

Organism

Organism UniProt Comment Textmining
Eubacterium sp.
-
-
-
Eubacterium sp. 144
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
16alpha-Hydroxyprogesterone r Eubacterium sp. 16-Dehydroprogesterone
-
?
16alpha-Hydroxyprogesterone in the reverse reaction the enzyme is only 15% as active as in the forward reaction Eubacterium sp. 16-Dehydroprogesterone
-
?
16alpha-Hydroxyprogesterone r Eubacterium sp. 144 16-Dehydroprogesterone
-
?
16alpha-Hydroxyprogesterone in the reverse reaction the enzyme is only 15% as active as in the forward reaction Eubacterium sp. 144 16-Dehydroprogesterone
-
?
16alpha-Hydroxypregnenolone activity is slight less than with 16alpha-hydroxyprogesterone Eubacterium sp. ?
-
?
16alpha-Hydroxypregnenolone activity is slight less than with 16alpha-hydroxyprogesterone Eubacterium sp. 144 ?
-
?
DELTA16-Progesterone
-
Eubacterium sp. ?
-
?
DELTA16-Progesterone
-
Eubacterium sp. 144 ?
-
?
16alpha-hydroxypregnenolone
-
Eubacterium sp. 16-dehydropregnenolone + H2O
-
?
16alpha-hydroxypregnenolone
-
Eubacterium sp. 144 16-dehydropregnenolone + H2O
-
?
16alpha-hydroxyprogesterone in the hydration direction the enzyme is only 15% as active as in the dehdyration direction Eubacterium sp. DELTA16-progesterone + H2O
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
Eubacterium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 50 35°C: about 65% of maximal activity, 50°C: about 40% of maximal activity Eubacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
6 min, 70% loss of activity Eubacterium sp.
65
-
10 min, 70% inactivation Eubacterium sp.
60
-
10 min, stable Eubacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8 8 the enzyme exhibits a broad pH range without a distinct pH-optimum Eubacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.8 8 broad pH-range without a distinct pH-optimum Eubacterium sp.
5.8 8 the enzyme exhibits a broad pH range without a distinct pH-optimum. Above pH 8.5 the activity decreases and is undetectable at pH 10.0 Eubacterium sp.