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Literature summary for 4.2.1.96 extracted from

  • Cameron, S.; Fyffe, S.A.; Goldie, S.; Hunter, W.N.
    Crystal structures of Toxoplasma gondii pterin-4a-carbinolamine dehydratase and comparisons with mammalian and parasite orthologues (2008), Mol. Biochem. Parasitol., 158, 131-138.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3). There is a high degree of structural similarity between Toxoplasma gondii PCD and the mammalian orthologue, Rattus norvegicus PCD, which is identical to the human enzyme Toxoplasma gondii

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion, 2V6S: 1.4 M ammonium sulfate, 1 M lithium sulfate, 2 mM 7,8-dihydrobiopterin, pH 7.6, space group P 43 21 2, resolution 2.22 A, complex with substrate-like ligand 7,8-dihydrobiopterin TgPCD:BH2 complex 2V6T: 0.4 M potassium-sodium tartrate, pH 7.6, space group P 43 21 2, resolution 3.1 A, apo-enzyme 2V6U: 1.4 M ammonium sulfate, 1 M lithium sulfate, pH 7.6, space group P 43 21 2, resolution 1.6 A Toxoplasma gondii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24200
-
asymmetric unit contains two subunits 2 * 24200 Toxoplasma gondii
46000
-
gel filtration Toxoplasma gondii

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii Q2Q449
-
-

Purification (Commentary)

Purification (Comment) Organism
by affinity chromatography over a HisTrap nickel chelating column, followed by TEV cleavage/dialysis at 4°C overnight and then further passage over the a HisTrap nickel column to remove the tag, contaminants and the His-tagged TEV protease. A final purification step using a Superdex 75 size-exclusion column is performed Toxoplasma gondii

Subunits

Subunits Comment Organism
tetramer asymmetric unit contains two subunits 2 * 24200 Toxoplasma gondii

Synonyms

Synonyms Comment Organism
PCD
-
Toxoplasma gondii
Pterin-4a-carbinolamine dehydratase
-
Toxoplasma gondii