Cloned (Comment) | Organism |
---|---|
gene SS1G_13314, sequence comparisons, phylogenetic tree, semiquantitative RT-PCR expression analysis | Sclerotinia sclerotiorum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | gene SCD1 encoding a scytalone dehydratase is disrupted by split-marker target gene replacement, protoplast transformation. Disruption of SCD1 does not change the pathogenicity of the fungus, but results in slower radial growth, less biomass, wider angled hyphal branches, impaired sclerotial development and decreased resistance to UV light. All gene knockout strains cause pathogenicity symptoms similar to those of the wild-type with both inoculation methods | Sclerotinia sclerotiorum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Sclerotinia sclerotiorum | pathogenicity assays are conducted on wounded and unwounded leaves of rapeseed seedlings. All gene knockout strains cause symptoms similar to those of the wild-type with both inoculation methods | ? | - |
? | |
additional information | Sclerotinia sclerotiorum ATCC 18683 / 1980 / Ss-1 | pathogenicity assays are conducted on wounded and unwounded leaves of rapeseed seedlings. All gene knockout strains cause symptoms similar to those of the wild-type with both inoculation methods | ? | - |
? | |
scytalone | Sclerotinia sclerotiorum | - |
1,3,8-trihydroxynaphthalene + H2O | - |
r | |
scytalone | Sclerotinia sclerotiorum ATCC 18683 / 1980 / Ss-1 | - |
1,3,8-trihydroxynaphthalene + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sclerotinia sclerotiorum | A7F6T4 | i.e. Whetzelinia sclerotiorum, isolated from diseased rapeseed (Brassica napus cv. Westar) in a field | - |
Sclerotinia sclerotiorum ATCC 18683 / 1980 / Ss-1 | A7F6T4 | i.e. Whetzelinia sclerotiorum, isolated from diseased rapeseed (Brassica napus cv. Westar) in a field | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the expression level of SCD1 is significantly (P < 0.05) higher in sclerotia than in mycelia at all developmental stages, whereas the expression in mycelia does not change between stages. SCD1 expression in sclerotia is significantly upregulated relative to that in mycelia in the matured stage | Sclerotinia sclerotiorum | - |
mycelium | - |
Sclerotinia sclerotiorum | - |
sclerotium | - |
Sclerotinia sclerotiorum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | pathogenicity assays are conducted on wounded and unwounded leaves of rapeseed seedlings. All gene knockout strains cause symptoms similar to those of the wild-type with both inoculation methods | Sclerotinia sclerotiorum | ? | - |
? | |
additional information | pathogenicity assays are conducted on wounded and unwounded leaves of rapeseed seedlings. All gene knockout strains cause symptoms similar to those of the wild-type with both inoculation methods | Sclerotinia sclerotiorum ATCC 18683 / 1980 / Ss-1 | ? | - |
? | |
scytalone | - |
Sclerotinia sclerotiorum | 1,3,8-trihydroxynaphthalene + H2O | - |
r | |
scytalone | - |
Sclerotinia sclerotiorum ATCC 18683 / 1980 / Ss-1 | 1,3,8-trihydroxynaphthalene + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | a SCD1 three-dimensional structural model of the hypothetical proteins is constructed on the basis of domain homology of the deduced amino acid sequence, which primarily provides the detailed structural analysis models representing the secondary structure, oligo-state without any ligands | Sclerotinia sclerotiorum |
Synonyms | Comment | Organism |
---|---|---|
SCD1 | - |
Sclerotinia sclerotiorum |
SS1G_13314 | - |
Sclerotinia sclerotiorum |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic analyses reveal that Scd1 sequences of Sclerotinia sclerotiorum and Botrytis cinerea form a monophyletic group | Sclerotinia sclerotiorum |
malfunction | deficiency of the melanin biosynthesis genes SCD1 (encoding enzyme scytalone dehydratase) and THR1 (encoding a trihydroxynaphthalene reductase) affects sclerotial development and vegetative growth, but not pathogenicity, in Sclerotinia sclerotiorum | Sclerotinia sclerotiorum |
metabolism | the enzyme is involved in melanin biosynthesis | Sclerotinia sclerotiorum |
physiological function | role of the enzyme in mycelial growth, sclerotial development and fungal pathogenicity. SCD1 contributes to the initial formation of sclerotia | Sclerotinia sclerotiorum |