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Literature summary for 4.2.1.94 extracted from

  • Liang, Y.; Xiong, W.; Steinkellner, S.; Feng, J.
    Deficiency of the melanin biosynthesis genes SCD1 and THR1 affects sclerotial development and vegetative growth, but not pathogenicity, in Sclerotinia sclerotiorum (2018), Mol. Plant Pathol., 19, 1444-1453 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene SS1G_13314, sequence comparisons, phylogenetic tree, semiquantitative RT-PCR expression analysis Sclerotinia sclerotiorum

Protein Variants

Protein Variants Comment Organism
additional information gene SCD1 encoding a scytalone dehydratase is disrupted by split-marker target gene replacement, protoplast transformation. Disruption of SCD1 does not change the pathogenicity of the fungus, but results in slower radial growth, less biomass, wider angled hyphal branches, impaired sclerotial development and decreased resistance to UV light. All gene knockout strains cause pathogenicity symptoms similar to those of the wild-type with both inoculation methods Sclerotinia sclerotiorum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Sclerotinia sclerotiorum pathogenicity assays are conducted on wounded and unwounded leaves of rapeseed seedlings. All gene knockout strains cause symptoms similar to those of the wild-type with both inoculation methods ?
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?
additional information Sclerotinia sclerotiorum ATCC 18683 / 1980 / Ss-1 pathogenicity assays are conducted on wounded and unwounded leaves of rapeseed seedlings. All gene knockout strains cause symptoms similar to those of the wild-type with both inoculation methods ?
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?
scytalone Sclerotinia sclerotiorum
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1,3,8-trihydroxynaphthalene + H2O
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r
scytalone Sclerotinia sclerotiorum ATCC 18683 / 1980 / Ss-1
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1,3,8-trihydroxynaphthalene + H2O
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r

Organism

Organism UniProt Comment Textmining
Sclerotinia sclerotiorum A7F6T4 i.e. Whetzelinia sclerotiorum, isolated from diseased rapeseed (Brassica napus cv. Westar) in a field
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Sclerotinia sclerotiorum ATCC 18683 / 1980 / Ss-1 A7F6T4 i.e. Whetzelinia sclerotiorum, isolated from diseased rapeseed (Brassica napus cv. Westar) in a field
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Source Tissue

Source Tissue Comment Organism Textmining
additional information the expression level of SCD1 is significantly (P < 0.05) higher in sclerotia than in mycelia at all developmental stages, whereas the expression in mycelia does not change between stages. SCD1 expression in sclerotia is significantly upregulated relative to that in mycelia in the matured stage Sclerotinia sclerotiorum
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mycelium
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Sclerotinia sclerotiorum
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sclerotium
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Sclerotinia sclerotiorum
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information pathogenicity assays are conducted on wounded and unwounded leaves of rapeseed seedlings. All gene knockout strains cause symptoms similar to those of the wild-type with both inoculation methods Sclerotinia sclerotiorum ?
-
?
additional information pathogenicity assays are conducted on wounded and unwounded leaves of rapeseed seedlings. All gene knockout strains cause symptoms similar to those of the wild-type with both inoculation methods Sclerotinia sclerotiorum ATCC 18683 / 1980 / Ss-1 ?
-
?
scytalone
-
Sclerotinia sclerotiorum 1,3,8-trihydroxynaphthalene + H2O
-
r
scytalone
-
Sclerotinia sclerotiorum ATCC 18683 / 1980 / Ss-1 1,3,8-trihydroxynaphthalene + H2O
-
r

Subunits

Subunits Comment Organism
More a SCD1 three-dimensional structural model of the hypothetical proteins is constructed on the basis of domain homology of the deduced amino acid sequence, which primarily provides the detailed structural analysis models representing the secondary structure, oligo-state without any ligands Sclerotinia sclerotiorum

Synonyms

Synonyms Comment Organism
SCD1
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Sclerotinia sclerotiorum
SS1G_13314
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Sclerotinia sclerotiorum

General Information

General Information Comment Organism
evolution phylogenetic analyses reveal that Scd1 sequences of Sclerotinia sclerotiorum and Botrytis cinerea form a monophyletic group Sclerotinia sclerotiorum
malfunction deficiency of the melanin biosynthesis genes SCD1 (encoding enzyme scytalone dehydratase) and THR1 (encoding a trihydroxynaphthalene reductase) affects sclerotial development and vegetative growth, but not pathogenicity, in Sclerotinia sclerotiorum Sclerotinia sclerotiorum
metabolism the enzyme is involved in melanin biosynthesis Sclerotinia sclerotiorum
physiological function role of the enzyme in mycelial growth, sclerotial development and fungal pathogenicity. SCD1 contributes to the initial formation of sclerotia Sclerotinia sclerotiorum