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Literature summary for 4.2.1.91 extracted from
- Subcellular localization of Arabidopsis arogenate dehydratases suggests novel and non-enzymatic roles (2017), J. Exp. Bot., 68, 1425-1440 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ADT1, recombinant expression of C-terminally CFP-tagged enzyme in Nicotiana benthamiana under control of the CaMV 35S promoter. Transient expression pf CFP-tagged ADT1 in Arabidopsis thaliana | Arabidopsis thaliana |
| gene ADT2, recombinant expression of C-terminally CFP-tagged enzyme in Nicotiana benthamiana under control of the CaMV 35S promoter. Transient expression pf CFP-tagged ADT2 in Arabidopsis thaliana | Arabidopsis thaliana |
| gene ADT3, recombinant expression of C-terminally CFP-tagged enzyme in Nicotiana benthamiana under control of the CaMV 35S promoter. Transient expression pf CFP-tagged ADT3 in Arabidopsis thaliana | Arabidopsis thaliana |
| gene ADT4, recombinant expression of C-terminally CFP-tagged enzyme in Nicotiana benthamiana under control of the CaMV 35S promoter. Transient expression pf CFP-tagged ADT4 in Arabidopsis thaliana | Arabidopsis thaliana |
| gene ADT5, recombinant expression of C-terminally CFP-tagged enzyme in Nicotiana benthamiana under control of the CaMV 35S promoter. CFP-tagged ADT5 is co-infiltrated with a YFP fusion to the nuclear marker NUP1, a component of the nuclear pore complex in Arabidopsis thaliana that has previously been shown to localize to the nuclear membrane. Transient expression pf CFP-tagged ADT5 in Arabidopsis thaliana | Arabidopsis thaliana |
| gene ADT6, recombinant expression of C-terminally CFP-tagged enzyme in Nicotiana benthamiana under control of the CaMV 35S promoter. Transient expression pf CFP-tagged ADT6 in Arabidopsis thaliana | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | chloroplast morphology and FtsZ2-YFP localization is affected by a point mutation in ADT2 mutant adt2-1D | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | ADT1 localizes to stroma and to areas seemingly close to the chloroplast just outside of the autofluorescence signal generated by chlorophyll. CFP-tagged ADT1-ADT5 localize to stroma and to areas seemingly close to the chloroplast just outside of the autofluorescence signal generated by chlorophyll. They often appear either in thread-like structures (e.g. the arrow in ADT2) or globular structures (e.g. the arrows in ADT4). The CFP-ADT6 pattern is distinctly different, showing a cytosolic distribution | Arabidopsis thaliana | 9507 | - |
| chloroplast | ADT2 localizes to stroma and to areas seemingly close to the chloroplast just outside of the autofluorescence signal generated by chlorophyll. CFP-tagged ADT1-ADT5 localize to stroma and to areas seemingly close to the chloroplast just outside of the autofluorescence signal generated by chlorophyll. They often appear either in thread-like structures (e.g. the arrow in ADT2) or globular structures (e.g. the arrows in ADT4). The CFP-ADT6 pattern is distinctly different, showing a cytosolic distribution. ADT2 forms structures consistent with chloroplast division rings. In addition, ADT2 accumulates in a spindle-like shape that tapers at chloroplast poles. This fusiform ADT2 accumulation is only found at one pole of the chloroplast and is distinct from a stromule pattern | Arabidopsis thaliana | 9507 | - |
| chloroplast | ADT3 localizes to stroma and to areas seemingly close to the chloroplast just outside of the autofluorescence signal generated by chlorophyll. CFP-tagged ADT1-ADT5 localize to stroma and to areas seemingly close to the chloroplast just outside of the autofluorescence signal generated by chlorophyll. They often appear either in thread-like structures (e.g. the arrow in ADT2) or globular structures (e.g. the arrows in ADT4). The CFP-ADT6 pattern is distinctly different, showing a cytosolic distribution | Arabidopsis thaliana | 9507 | - |
| chloroplast | ADT4 localizes to stroma and to areas seemingly close to the chloroplast just outside of the autofluorescence signal generated by chlorophyll. CFP-tagged ADT1-ADT5 localize to stroma and to areas seemingly close to the chloroplast just outside of the autofluorescence signal generated by chlorophyll. They often appear either in thread-like structures (e.g. the arrow in ADT2) or globular structures (e.g. the arrows in ADT4). The CFP-ADT6 pattern is distinctly different, showing a cytosolic distribution | Arabidopsis thaliana | 9507 | - |
| chloroplast | ADT5 localizes to stroma and to areas seemingly close to the chloroplast just outside of the autofluorescence signal generated by chlorophyll. CFP-tagged ADT1-ADT5 localize to stroma and to areas seemingly close to the chloroplast just outside of the autofluorescence signal generated by chlorophyll. They often appear either in thread-like structures (e.g. the arrow in ADT2) or globular structures (e.g. the arrows in ADT4). The CFP-ADT6 pattern is distinctly different, showing a cytosolic distribution | Arabidopsis thaliana | 9507 | - |
| cytosol | the CFP-ADT6 pattern shows a cytosolic distribution | Arabidopsis thaliana | 5829 | - |
| additional information | isozyme localization study, expression analysis of fluorescence-tagged ADT isozymes, overview. CFP-tagged ADT1-ADT5 localize to stroma and to areas seemingly close to the chloroplast just outside of the autofluorescence signal generated by chlorophyll. They often appear either in thread-like structures (e.g. the arrow in ADT2) or globular structures (e.g. the arrows in ADT4). The CFP-ADT6 pattern is distinctly different, showing a cytosolic distribution | Arabidopsis thaliana | - |
- |
| additional information | isozyme localization study, expression analysis of fluorescence-tagged ADT isozymes, overview. CFP-tagged ADT1-ADT5 localize to stroma and to areas seemingly close to the chloroplast just outside of the autofluorescence signal generated by chlorophyll. They often appear either in thread-like structures (e.g. the arrow in ADT2) or globular structures (e.g. the arrows in ADT4). The CFP-ADT6 pattern is distinctly different, showing a cytosolic distribution. In addition to its chloroplast localization, only CFP-ADT5 is also detected in nuclei | Arabidopsis thaliana | - |
- |
| additional information | isozyme localization study, expression analysis of fluorescence-tagged ADT isozymes, overview. CFP-tagged ADT1-ADT5 localize to stroma and to thread-like structures that are as seemingly close to the chloroplast just outside of the autofluorescence signal generated by chlorophyll. They often appear either in thread-like structures (e.g. the arrow in ADT2) or globular structures (e.g. the arrows in ADT4). The CFP-ADT6 pattern is distinctly different, showing a cytosolic distribution | Arabidopsis thaliana | - |
- |
| nucleus | ADT5 proteins are unique as they are the only full-length ADT proteins that are found in the nucleus | Arabidopsis thaliana | 5634 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arogenate | Arabidopsis thaliana | - |
L-phenylalanine + H2O + CO2 | - |
? |  |
| L-arogenate | Arabidopsis thaliana Col-0 | - |
L-phenylalanine + H2O + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9FNJ8 | - |
- |
| Arabidopsis thaliana | Q9SA96 | - |
- |
| Arabidopsis thaliana | Q9SGD6 | termed chloroplastic, but the CFP-ADT6 pattern shows a cytosolic distribution | - |
| Arabidopsis thaliana | Q9SSE7 | - |
- |
| Arabidopsis thaliana | Q9ZUY3 | - |
- |
| Arabidopsis thaliana Col-0 | Q9FNJ8 | - |
- |
| Arabidopsis thaliana Col-0 | Q9SA96 | - |
- |
| Arabidopsis thaliana Col-0 | Q9SGD6 | termed chloroplastic, but the CFP-ADT6 pattern shows a cytosolic distribution | - |
| Arabidopsis thaliana Col-0 | Q9SSE7 | - |
- |
| Arabidopsis thaliana Col-0 | Q9ZUY3 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arogenate | - |
Arabidopsis thaliana | L-phenylalanine + H2O + CO2 | - |
? | |
| L-arogenate | - |
Arabidopsis thaliana Col-0 | L-phenylalanine + H2O + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AdT | - |
Arabidopsis thaliana |
| ADT1 | - |
Arabidopsis thaliana |
| ADT2 | - |
Arabidopsis thaliana |
| ADT3 | - |
Arabidopsis thaliana |
| ADT4 | - |
Arabidopsis thaliana |
| ADT5 | - |
Arabidopsis thaliana |
| ADT6 | - |
Arabidopsis thaliana |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | chloroplast morphology and FtsZ2-YFP localization is affected by a point mutation in ADT2 in mutant adt2-1D | Arabidopsis thaliana |
| additional information | ADT2 forms structures consistent with chloroplast division rings | Arabidopsis thaliana |
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