Cloned (Comment) | Organism |
---|---|
coding regions corresponding to mature ADT protein (lacking the N-terminal plastid transit peptides) subcloned into the expression vector pET-28a, which contains an N-terminal 6xHis tag. Recombinant protein expressed in Escherichia coli Rosetta cells | Petunia x hybrida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0488 | - |
L-arogenate | at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA | Petunia x hybrida | |
0.0667 | - |
L-arogenate | at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA | Petunia x hybrida | |
0.179 | - |
L-arogenate | at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA | Petunia x hybrida | |
0.4658 | - |
prephenate | at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA | Petunia x hybrida | |
0.752 | - |
prephenate | at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA | Petunia x hybrida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Petunia x hybrida | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43400 | - |
sequence analysis | Petunia x hybrida |
45900 | - |
sequence analysis | Petunia x hybrida |
47300 | - |
sequence analysis | Petunia x hybrida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Petunia x hybrida | D3U715 | - |
- |
Petunia x hybrida | D3U716 | - |
- |
Petunia x hybrida | D3U717 | - |
- |
Purification (Comment) | Organism |
---|---|
by fast protein liquid chromatography and gel filtration | Petunia x hybrida |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Petunia x hybrida | - |
leaf | - |
Petunia x hybrida | - |
petal | - |
Petunia x hybrida | - |
stem | only ADT1 and ADT3 | Petunia x hybrida | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arogenate | - |
Petunia x hybrida | L-phenylalanine + CO2 + H2O | - |
? | |
L-arogenate | ADT1 shows strict substrate specificity toward arogenate, although with the lowest catalytic efficiency among the three ADTs | Petunia x hybrida | L-phenylalanine + CO2 + H2O | - |
? | |
prephenate | - |
Petunia x hybrida | phenylpyruvate + CO2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADT1 | - |
Petunia x hybrida |
ADT2 | - |
Petunia x hybrida |
ADT3 | - |
Petunia x hybrida |
arogenate dehydratase1 | - |
Petunia x hybrida |
arogenate dehydratase2 | - |
Petunia x hybrida |
arogenate dehydratase3 | - |
Petunia x hybrida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
prephenate | at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA | Petunia x hybrida | |
0.06 | - |
prephenate | at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA | Petunia x hybrida | |
0.194 | - |
L-arogenate | at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA | Petunia x hybrida | |
0.267 | - |
L-arogenate | at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA | Petunia x hybrida | |
1.231 | - |
L-arogenate | at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA | Petunia x hybrida |
Organism | Comment | Expression |
---|---|---|
Petunia x hybrida | ADT1 suppression via RNA interference in petunia petals significantly reduces ADT activity, levels of Phe, and downstream phenylpropanoid/benzenoid volatiles, whereby arogenate levels are unaltered, while shikimate and Trp levels are decreased in transgenic petals | down |
Petunia x hybrida | ADT1 exhibits the highest level of expression in corolla and tube, the scentproducing parts of petunia flowers. In corolla, the ADT1 transcript levels exceed those of ADT2 and ADT3 by 88- and 10fold, respectively. ADT1 expression is positively correlated with endogenous Phe levels throughout flower development | up |
Petunia x hybrida | in corolla, the ADT1 transcript levels exceed those of ADT2 and ADT3 by 88- and 10fold, respectively | up |
General Information | Comment | Organism |
---|---|---|
malfunction | ADT1 suppression leads to downregulation of carbon flux toward shikimic acid, but exogenous supply of shikimate bypasses this negative regulation and results in elevated arogenate accumulation | Petunia x hybrida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
prephenate | at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA | Petunia x hybrida | |
0.08 | - |
prephenate | at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA | Petunia x hybrida | |
1.48 | - |
L-arogenate | at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA | Petunia x hybrida | |
4 | - |
L-arogenate | at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA | Petunia x hybrida | |
18.51 | - |
L-arogenate | at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA | Petunia x hybrida |