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Literature summary for 4.2.1.91 extracted from

  • Zhao, G.; Xia, T.; Fischer, R.S.; Jensen, R.A.
    Cyclohexadienyl dehydratase from Pseudomonas aeruginosa. Molecular cloning of the gene and characterization of the gene product (1992), J. Biol. Chem., 267, 2487-2493.
    View publication on PubMed
Search for more literature for 4.2.1.91

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas aeruginosa
expression in Escherichia coli Pseudomonas aeruginosa
pheC cloned and expressed by functional complementation of a pheA auxotroph of Escherichia coli Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
L-arogenate competitive inhibition of prephenate dehydratase activity; competitive to prephenate; reaction with prephenate is inhibited Pseudomonas aeruginosa
prephenate competitive inhibition; competitive to L-arogenate; reaction with L-arogenate is inhibited Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 L-arogenate enzyme purified directly from Pseudomonas aeruginosa Pseudomonas aeruginosa
0.22
-
 L-arogenate pH 7.5, 37°C Pseudomonas aeruginosa
0.22
-
 L-arogenate pH 7.5, 22°C Pseudomonas aeruginosa
0.22
-
 L-arogenate recombinant protein, purified from Escherichia coli Pseudomonas aeruginosa
0.4
-
 prephenate enzyme purified directly from Pseudomonas aeruginosa Pseudomonas aeruginosa
0.42
-
 prephenate pH 7.5, 37°C Pseudomonas aeruginosa
0.42
-
 prephenate pH 7.5, 22°C Pseudomonas aeruginosa
0.42
-
 prephenate recombinant protein, purified from Escherichia coli Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29500
-
 2 * 29500, SDS-PAGE, homodimer Pseudomonas aeruginosa
29500
-
 2 * 29500, SDS-PAGE, gel filtration Pseudomonas aeruginosa
29500
-
 2 * 30480, calculated, 2 * 29500, SDS-PAGE Pseudomonas aeruginosa
30480
-
 calculated from cloned gene pheC Pseudomonas aeruginosa
30480
-
 2 * 30480, calculated from amino acis sequence Pseudomonas aeruginosa
72000
-
 gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arogenate Pseudomonas aeruginosa biosynthetic pathway of phenylalanine L-phenylalanine + CO2 + H2O
-
 ?
L-arogenate Pseudomonas aeruginosa
-
 L-phenylalanine + H2O + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa Q01269
-
-

Purification (Commentary)

Purification (Comment) Organism
native and recombinant enzyme Pseudomonas aeruginosa
recombinant protein Pseudomonas aeruginosa
to electrophoretic homogeneity Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0483
-
 L-arogenate dehydratase activity Pseudomonas aeruginosa
0.158
-
 prephenate dehydratase activity Pseudomonas aeruginosa
102.8
-
 purified recombinant enzyme, with L-arogenate as substrate Pseudomonas aeruginosa
307.7
-
 purified recombinant enzyme, with prephenate as substrate Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arogenate biosynthetic pathway of phenylalanine Pseudomonas aeruginosa L-phenylalanine + CO2 + H2O
-
 ?
L-arogenate
-
 Pseudomonas aeruginosa L-phenylalanine + H2O + CO2
-
 ?
additional information specific activity ratio of prephenate dehydratase to arogenate dehydratase activity is 3:1 Pseudomonas aeruginosa ?
-
 ?
prephenate pH 7.5, 37°C Pseudomonas aeruginosa phenylpyruvate + H2O + CO2
-
 ?
prephenate
-
 Pseudomonas aeruginosa phenylpyruvate + CO2 + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 29500, SDS-PAGE, homodimer Pseudomonas aeruginosa
dimer 2 * 29500, SDS-PAGE, gel filtration Pseudomonas aeruginosa
dimer 2 * 30480, calculated from amino acis sequence Pseudomonas aeruginosa
dimer 2 * 30480, calculated, 2 * 29500, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
arogenate dehydratase
-
 Pseudomonas aeruginosa
cyclohexadienyl dehydratase
-
 Pseudomonas aeruginosa
prephenate dehydratase
-
 Pseudomonas aeruginosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2
-
 L-arogenate
-
 Pseudomonas aeruginosa
0.2
-
 L-arogenate pH 7.5, 37°C Pseudomonas aeruginosa
0.2
-
 L-arogenate pH 7.5, 22°C Pseudomonas aeruginosa
0.4
-
 prephenate
-
 Pseudomonas aeruginosa
0.4
-
 prephenate pH 7.5, 37°C Pseudomonas aeruginosa
0.4
-
 prephenate pH 7.5, 22°C Pseudomonas aeruginosa