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Literature summary for 4.2.1.9 extracted from

  • Holatko, J.; Elisakova, V.; Prouza, M.; Sobotka, M.; Nesvera, J.; Patek, M.
    Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation (2009), J. Biotechnol., 139, 203-210.
    View publication on PubMed
Search for more literature for 4.2.1.9

Cloned(Commentary)

Cloned (Comment) Organism
gene ilvD, expression in Escherichia coli DH5alpha, overexpression Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
additional information construction of a valine producer by genetic engineering, mutant strains are resistant against inhibition by branched-chain amino acids L-valine, L-isoleucine and L-leucine, A constructed weak promoter of ilvA or leuA, which is introduced into the Corynebacterium glutamicum chromosome via a gene-replacement technique, reduces the biosynthetic rate of isoleucine or leucine, which lowers the mutant growth rate and increases valine production. Overexpression of ilvD driven by the strong mutant promoter P-ilvDM7 resultsin an even higher level of valine production, engineered strains, overview Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,3-dihydroxy-3-methylvalerate Corynebacterium glutamicum
-
 3-methyl-2-oxovalerate + H2O
-
 ?
2,3-dihydroxy-isovalerate Corynebacterium glutamicum
-
 2-oxoisovalerate
-
 ?

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
 gene ilvD, strain ilvNM13, ATCC 13032
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydroxy-3-methylvalerate
-
 Corynebacterium glutamicum 3-methyl-2-oxovalerate + H2O
-
 ?
2,3-dihydroxy-isovalerate
-
 Corynebacterium glutamicum 2-oxoisovalerate
-
 ?

Synonyms

Synonyms Comment Organism
DHAD
-
 Corynebacterium glutamicum
dihydroxyacid dehydratase
-
 Corynebacterium glutamicum
IlvD
-
 Corynebacterium glutamicum