Any feedback?
Literature summary for 4.2.1.9 extracted from
- Characterization of enzymes of the branched-chain amino acid biosynthetic pathway in Methanococcus spp (1991), J. Bacteriol., 173, 2086-2092.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | strong | Methanococcus aeolicus |  |
| KCN | - |
Methanococcus aeolicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.8 | - |
2,3-dihydroxyisovalerate | - |
Methanococcus aeolicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | divalent cation required. Mg2+, Mn2+, Ni2+, Co2+, and Fe2+ are nearly equally effective | Methanococcus aeolicus | |
| Fe2+ | divalent cation required. Mg2+, Mn2+, Ni2+, Co2+, and Fe2+ are nearly equally effective | Methanococcus aeolicus | |
| Mg2+ | divalent cation required. Mg2+, Mn2+, Ni2+, Co2+, and Fe2+ are nearly equally effective | Methanococcus aeolicus | |
| Mn2+ | divalent cation required. Mg2+, Mn2+, Ni2+, Co2+, and Fe2+ are nearly equally effective | Methanococcus aeolicus | |
| Ni2+ | divalent cation required. Mg2+, Mn2+, Ni2+, Co2+, and Fe2+ are nearly equally effective | Methanococcus aeolicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-dihydroxyisovalerate | Methanococcus aeolicus | - |
? | - |
? | |
| 2,3-dihydroxyisovalerate | Methanococcus maripaludis | - |
? | - |
? | |
| 2,3-dihydroxyisovalerate | Methanococcus voltae | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanococcus aeolicus | - |
- |
- |
| Methanococcus maripaludis | - |
- |
- |
| Methanococcus voltae | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| 5% O2, at 25°C, half-life: less than 6 min, under anaerobic conditions the enzyme is stable for more than 8 days | Methanococcus aeolicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.63 | - |
- |
Methanococcus aeolicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-dihydroxy-3-methylbutanoate | - |
Methanococcus aeolicus | 2-oxoisovalerate + H2O | - |
? | |
| 2,3-dihydroxy-3-methylbutanoate | - |
Methanococcus maripaludis | 2-oxoisovalerate + H2O | - |
? | |
| 2,3-dihydroxy-3-methylbutanoate | - |
Methanococcus voltae | 2-oxoisovalerate + H2O | - |
? | |
| 2,3-dihydroxy-3-methylpentanoate | - |
Methanococcus aeolicus | 3-methyl-2-oxopentanoate + H2O | - |
? | |
| 2,3-dihydroxy-3-methylpentanoate | - |
Methanococcus maripaludis | 3-methyl-2-oxopentanoate + H2O | - |
? | |
| 2,3-dihydroxy-3-methylpentanoate | - |
Methanococcus voltae | 3-methyl-2-oxopentanoate + H2O | - |
? | |
| 2,3-dihydroxyisovalerate | - |
Methanococcus aeolicus | ? | - |
? | |
| 2,3-dihydroxyisovalerate | - |
Methanococcus maripaludis | ? | - |
? | |
| 2,3-dihydroxyisovalerate | - |
Methanococcus voltae | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Methanococcus aeolicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | pH 6.0: about 30% of maximal activity, pH 9.0: about 35% of maximal activity | Methanococcus aeolicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
