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Literature summary for 4.2.1.84 extracted from

  • Sun, S.; Lu, T.; Yang, W.; Guo, J.; Rui, X.; Mao, S.; Zhou, L.; Dai, Y.
    Characterization of a versatile nitrile hydratase of the neonicotinoid thiacloprid-degrading bacterium Ensifer meliloti CGMCC 7333 (2016), RSC Adv., 6, 15501-15508 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli rosetta Sinorhizobium meliloti

Inhibitors

Inhibitors Comment Organism Structure
acetone 2% (v/v), 70.3% inhibition Sinorhizobium meliloti
Cu2+ 0.1 mM, 14.8% inhibition Sinorhizobium meliloti
ethanol 2% (v/v), 68.6% inhibition Sinorhizobium meliloti
ethyl acetate 2% (v/v), 76% inhibition Sinorhizobium meliloti
Fe2+ 0.1 mM, 8% inhibition Sinorhizobium meliloti
isoamyl alcohol 2% (v/v), 29.9% inhibition Sinorhizobium meliloti
Isopropanol 2% (v/v), 35.7% inhibition Sinorhizobium meliloti
methanol 2% (v/v), 19.2% inhibition Sinorhizobium meliloti
Zn2+ 0.1 mM, 8% inhibition Sinorhizobium meliloti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
acetamiprid pH 7.5, 30°C Sinorhizobium meliloti
7.34
-
3-Cyanopyridine pH 7.5, 30°C Sinorhizobium meliloti
12.39
-
thiacloprid pH 7.5, 30°C Sinorhizobium meliloti
14.81
-
Benzonitrile pH 7.5, 30°C Sinorhizobium meliloti
17.91
-
indole-3-acetonitrile pH 7.5, 30°C Sinorhizobium meliloti

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ cobalt-type nitrile hydratase, and the addition of cobalt to the cell growth broth significantly stimulates nitrile hydratase activity. Supplementing cobalt to phosphate buffer containing purified enzyme has no effect on nitrile hydratase activity Sinorhizobium meliloti

Organism

Organism UniProt Comment Textmining
Sinorhizobium meliloti V5RFK1 AND V5RE14
-
-
Sinorhizobium meliloti CGMCC 7333 V5RFK1 AND V5RE14
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sinorhizobium meliloti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-cyanopyridine + H2O hydration at 5528% compared to the activity with thiacloprid Sinorhizobium meliloti pyridine-3-carbamide
-
?
3-cyanopyridine + H2O hydration at 5528% compared to the activity with thiacloprid Sinorhizobium meliloti CGMCC 7333 pyridine-3-carbamide
-
?
acetamiprid + H2O hydration at 22.41% compared to the activity with thiacloprid Sinorhizobium meliloti (1E)-N'-carbamoyl-N-[(6-chloropyridin-3-yl)methyl]-N-methylethanimidamide
-
?
acetonitrile + H2O hydration at 29.02% compared to the activity with thiacloprid Sinorhizobium meliloti acetamide
-
?
acetonitrile + H2O hydration at 29.02% compared to the activity with thiacloprid Sinorhizobium meliloti CGMCC 7333 acetamide
-
?
benzonitrile + H2O hydration at 2532% compared to the activity with thiacloprid Sinorhizobium meliloti benzamide
-
?
benzonitrile + H2O hydration at 2532% compared to the activity with thiacloprid Sinorhizobium meliloti CGMCC 7333 benzamide
-
?
hexanedinitrile + H2O hydration at 0.43% compared to the activity with thiacloprid Sinorhizobium meliloti ?
-
?
indole-3-acetonitrile + H2O hydration at 479.79% compared to the activity with thiacloprid Sinorhizobium meliloti (indole-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O hydration at 479.79% compared to the activity with thiacloprid Sinorhizobium meliloti CGMCC 7333 (indole-3-yl)acetamide
-
?
isobutyronitrile + H2O hydration at 3.78% compared to the activity with thiacloprid Sinorhizobium meliloti isobutyramide
-
?
succinonitrile + H2O hydration at 13.17% compared to the activity with thiacloprid Sinorhizobium meliloti ?
-
?
succinonitrile + H2O hydration at 13.17% compared to the activity with thiacloprid Sinorhizobium meliloti CGMCC 7333 ?
-
?
thiacloprid + H2O
-
Sinorhizobium meliloti 1-[(2Z)-3-[(6-chloropyridin-3-yl)methyl]-1,3-thiazolidin-2-ylidene]urea
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 50 hydration of thiacloprid Sinorhizobium meliloti

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 20°C: about 45% of maximal activity, 60°C: about 20% of maximal activity Sinorhizobium meliloti

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
1 h, stable up to Sinorhizobium meliloti
50
-
1 h, 85% loss of activity Sinorhizobium meliloti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.04
-
acetamiprid pH 7.5, 30°C Sinorhizobium meliloti
131.36
-
thiacloprid pH 7.5, 30°C Sinorhizobium meliloti
426.68
-
indole-3-acetonitrile pH 7.5, 30°C Sinorhizobium meliloti
1054.36
-
3-Cyanopyridine pH 7.5, 30°C Sinorhizobium meliloti
1489.82
-
Benzonitrile pH 7.5, 30°C Sinorhizobium meliloti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Sinorhizobium meliloti

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 60% of maximal activity, pH 9.0: about 50% of maximal activity Sinorhizobium meliloti

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 93% activity is retained after storage at pH 6.0-9.0 for 12 h Sinorhizobium meliloti

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.42
-
acetamiprid pH 7.5, 30°C Sinorhizobium meliloti
10.6
-
thiacloprid pH 7.5, 30°C Sinorhizobium meliloti
23.8
-
indole-3-acetonitrile pH 7.5, 30°C Sinorhizobium meliloti
100.9
-
Benzonitrile pH 7.5, 30°C Sinorhizobium meliloti
146.43
-
3-Cyanopyridine pH 7.5, 30°C Sinorhizobium meliloti