Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require the help of a P47K activator protein to be active | Pseudomonas sp. |
Cloned (Comment) | Organism |
---|---|
gene nthAB, DNA and amino acid sequence determination, analysis, and comparison, phylogenetic analysis and tree, expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | iron-type metalloenzyme | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
indole-3-nitrile + H2O | Pseudomonas sp. | - |
indole-3-acetamide | the nitrile hydratase produces only indole-3-acetamide, no indole-3-acetic acid | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | K9NKC3 | gene nthA, alpha-subunit; gene nthAB | - |
Pseudomonas sp. | K9NLN7 | gene nthB, beta-subunit; gene nthAB | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pseudomonas sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition | optimum growth temperature of the bacterium is 25°C to 30°C, but it is able to grow at 4°C | Pseudomonas sp. | - |
additional information | optimum growth temperature of the bacterium is 25°C to 30°C, but it is able to grow at 4°C | Pseudomonas sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
indole-3-nitrile + H2O | - |
Pseudomonas sp. | indole-3-acetamide | - |
? | |
indole-3-nitrile + H2O | - |
Pseudomonas sp. | indole-3-acetamide | the nitrile hydratase produces only indole-3-acetamide, no indole-3-acetic acid | ? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | alphabeta, nitrile hydratase alpha (NthA), and beta (NthB) subunits | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
NthAB | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
- |
Pseudomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
maximal activity at 4°C, over 50% of maximal activity at temperatures of 10 to 20°C, the activity decreases significantly as the temperature increases above 20°C | Pseudomonas sp. |
- |
20 | maximal activity at 4°C, over 50% of maximal activity at temperatures of 10 to 20°C, the activity decreases significantly as the temperature increases above 20°C | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
narrow optimum, about 20% of maximal activity at pH 7.0 and about 40% at pH 8.0, profile overview | Pseudomonas sp. |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is important in the indole-3-acetic acid biosynthesis pathway together with the nitrilase, EC 3.5.5.1, which produces indole-3-amide, but also indole-3-acetic acid , overview | Pseudomonas sp. |