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Literature summary for 4.2.1.84 extracted from

  • Cantarella, L.; Gallifuoco, A.; Malandra, A.; Martinkova, L.; Spera, A.; Cantarella, M.
    High-yield continuous production of nicotinic acid via nitrile hydratase-amidase cascade reactions using cascade CSMRs (2011), Enzyme Microb. Technol., 48, 345-350.
    View publication on PubMed

Application

Application Comment Organism
synthesis bioconversion of 3-cyanopyridine using the in situ nitrile hydratase-amidase cascade system of resting Microbacterium imperiale CBS 498-74 cells in an ultrafiltration-membrane reactor, carried out in continuously stirred tank UF-membrane bioreactors arranged in series, the reactor configuration enables both enzymes, involved in the cascade reaction, to work with optimized kinetics, without any purification, exploiting their differing temperature dependences, method optimization, overview Microbacterium imperiale

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-cyanopyridine + H2O Microbacterium imperiale NHase-AMase cascade system exploited in a continuous reactor configuration, including nitrile hydratase and amidase, EC 3.5.1.4, activity. Bioconversion to intermediate nicotinamide and further to nicotinic acid nicotinamide
-
?
3-cyanopyridine + H2O Microbacterium imperiale CBS 498-74 NHase-AMase cascade system exploited in a continuous reactor configuration, including nitrile hydratase and amidase, EC 3.5.1.4, activity. Bioconversion to intermediate nicotinamide and further to nicotinic acid nicotinamide
-
?

Organism

Organism UniProt Comment Textmining
Microbacterium imperiale
-
-
-
Microbacterium imperiale CBS 498-74
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-cyanopyridine + H2O
-
Microbacterium imperiale nicotinamide
-
?
3-cyanopyridine + H2O NHase-AMase cascade system exploited in a continuous reactor configuration, including nitrile hydratase and amidase, EC 3.5.1.4, activity. Bioconversion to intermediate nicotinamide and further to nicotinic acid Microbacterium imperiale nicotinamide
-
?
3-cyanopyridine + H2O
-
Microbacterium imperiale CBS 498-74 nicotinamide
-
?
3-cyanopyridine + H2O NHase-AMase cascade system exploited in a continuous reactor configuration, including nitrile hydratase and amidase, EC 3.5.1.4, activity. Bioconversion to intermediate nicotinamide and further to nicotinic acid Microbacterium imperiale CBS 498-74 nicotinamide
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5
-
bioconversion in cells in a bioreactor Microbacterium imperiale

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
bioconversion in cells in a bioreactor Microbacterium imperiale