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Literature summary for 4.2.1.84 extracted from

  • Cantarella, L.; Gallifuoco, A.; Malandra, A.; Martinkova, L.; Pasquarelli, F.; Spera, A.; Cantarella, M.
    Application of continuous stirred membrane reactor to 3-cyanopyridine bioconversion using the nitrile hydratase-amidase cascade system of Microbacterium imperiale CBS 498-74 (2010), Enzyme Microb. Technol., 47, 64-70.
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Application

Application Comment Organism
synthesis bioconversion of 3-cyanopyridine using the in situ nitrile hydratase-amidase cascade system of resting Microbacterium imperiale CBS 498-74 cells in an ultrafiltration-membrane reactor, operated in either batch or continuous mode, method optimization, overview Microbacterium imperiale

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-cyanopyridine + H2O Microbacterium imperiale NHase-AMase cascade system exploited in a continuous reactor configuration, including nitrile hydratase and amidase, EC 3.5.1.4, activity. Bioconversion to intermediate nicotinamide and further to nicotinic acid nicotinamide
-
 ?
3-cyanopyridine + H2O Microbacterium imperiale CBS 498-74 NHase-AMase cascade system exploited in a continuous reactor configuration, including nitrile hydratase and amidase, EC 3.5.1.4, activity. Bioconversion to intermediate nicotinamide and further to nicotinic acid nicotinamide
-
 ?

Organism

Organism UniProt Comment Textmining
Microbacterium imperiale
-
-
-
Microbacterium imperiale CBS 498-74
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-cyanopyridine + H2O
-
 Microbacterium imperiale nicotinamide
-
 ?
3-cyanopyridine + H2O NHase-AMase cascade system exploited in a continuous reactor configuration, including nitrile hydratase and amidase, EC 3.5.1.4, activity. Bioconversion to intermediate nicotinamide and further to nicotinic acid Microbacterium imperiale nicotinamide
-
 ?
3-cyanopyridine + H2O
-
 Microbacterium imperiale CBS 498-74 nicotinamide
-
 ?
3-cyanopyridine + H2O NHase-AMase cascade system exploited in a continuous reactor configuration, including nitrile hydratase and amidase, EC 3.5.1.4, activity. Bioconversion to intermediate nicotinamide and further to nicotinic acid Microbacterium imperiale CBS 498-74 nicotinamide
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Microbacterium imperiale

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Microbacterium imperiale