Application | Comment | Organism |
---|---|---|
synthesis | enzyme can be used in conjunction with a stereoselective amidase to synthesize ethyl (S)-4-chloro-3-hydroxybutyrate, an intermediate for a hypercholesterolemia drug, Atorvastatin | Rhodococcus erythropolis |
General Stability | Organism |
---|---|
the partially purified enzyme is stable in the presence of organic acids at higher temperatures | Rhodococcus erythropolis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 39% reduced activity at 1 mM | Rhodococcus erythropolis | |
2-mercaptoethanol | 9% reduced activity at 1 mM | Rhodococcus erythropolis | |
ammonium persulfate | 52% reduced activity at 1 mM | Rhodococcus erythropolis | |
cobalt chloride | 20% reduced activity at 1 mM | Rhodococcus erythropolis | |
copper sulfate | 95% reduced activity at 1 mM | Rhodococcus erythropolis | |
dithiothreitol | 18% reduced activity at 1 mM | Rhodococcus erythropolis | |
EDTA | 15% reduced activity at 1 mM | Rhodococcus erythropolis | |
Ferrous sulfate | 54% reduced activity at 1 mM | Rhodococcus erythropolis | |
hydrogen peroxide | 97% reduced activity at 1 mM | Rhodococcus erythropolis | |
iodoacetamide | 22% reduced activity at 1 mM | Rhodococcus erythropolis | |
Mercury chloride | 89% reduced activity at 1 mM | Rhodococcus erythropolis | |
PMSF | 83% reduced activity at 1 mM | Rhodococcus erythropolis | |
Silver nitrate | 82% reduced activity at 1 mM | Rhodococcus erythropolis | |
zinc chloride | 56% reduced activity at 1 mM | Rhodococcus erythropolis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | NHase N'4 is a Fe-type enzyme with a Cys109-Ser110-Leu111-Cys112-Ser113-Cys114 sequence | Rhodococcus erythropolis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22953 | - |
2 * 27000, alpha-subunit, + 2 * 29000, beta-subunit, alpha2beta2 structure, SDS-PAGE, 2 * 22953, alpha-subunit, + 2 * 23486, beta-subunit, alpha2beta2 structure, sequence calculation | Rhodococcus erythropolis |
23486 | - |
2 * 27000, alpha-subunit, + 2 * 29000, beta-subunit, alpha2beta2 structure, SDS-PAGE, 2 * 22953, alpha-subunit, + 2 * 23486, beta-subunit, alpha2beta2 structure, sequence calculation | Rhodococcus erythropolis |
27000 | - |
2 * 27000, alpha-subunit, + 2 * 29000, beta-subunit, alpha2beta2 structure, SDS-PAGE, 2 * 22953, alpha-subunit, + 2 * 23486, beta-subunit, alpha2beta2 structure, sequence calculation | Rhodococcus erythropolis |
29000 | - |
2 * 27000, alpha-subunit, + 2 * 29000, beta-subunit, alpha2beta2 structure, SDS-PAGE, 2 * 22953, alpha-subunit, + 2 * 23486, beta-subunit, alpha2beta2 structure, sequence calculation | Rhodococcus erythropolis |
92300 | - |
gel filtration | Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | - |
- |
- |
Rhodococcus erythropolis N4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 78fold by ammonium sulfate fractionation, anion exchange chromatography, and hydrophobic interaction chromatography | Rhodococcus erythropolis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
860 | - |
partially purified enzyme | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-chloro-3-hydroxybutyronitrile + H2O | the following reaction by an amidase leads to the correspondend carboxylic acid | Rhodococcus erythropolis | 4-chloro-3-hydroxybutyramide | - |
? | |
acetonitrile + H2O | very low activity | Rhodococcus erythropolis | acetamide | - |
? | |
acetonitrile + H2O | very low activity | Rhodococcus erythropolis N4 | acetamide | - |
? | |
acrylonitrile + H2O | - |
Rhodococcus erythropolis | 2-propenoic acid amide | i.e. acrylamide | ? | |
acrylonitrile + H2O | - |
Rhodococcus erythropolis N4 | 2-propenoic acid amide | i.e. acrylamide | ? | |
benzonitrile + H2O | low activity | Rhodococcus erythropolis | benzoic acid amide | - |
? | |
benzonitrile + H2O | low activity | Rhodococcus erythropolis N4 | benzoic acid amide | - |
? | |
butyronitrile + H2O | best substrate | Rhodococcus erythropolis | butyric acid amide | - |
? | |
isobutyronitrile + H2O | - |
Rhodococcus erythropolis | isobutyric acid amide | - |
? | |
isobutyronitrile + H2O | - |
Rhodococcus erythropolis N4 | isobutyric acid amide | - |
? | |
methacrylonitrile + H2O | - |
Rhodococcus erythropolis | methacrylic acid amide | - |
? | |
propionitrile + H2O | - |
Rhodococcus erythropolis | propionic acid amide | - |
? | |
propionitrile + H2O | - |
Rhodococcus erythropolis N4 | propionic acid amide | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 27000, alpha-subunit, + 2 * 29000, beta-subunit, alpha2beta2 structure, SDS-PAGE, 2 * 22953, alpha-subunit, + 2 * 23486, beta-subunit, alpha2beta2 structure, sequence calculation | Rhodococcus erythropolis |
Synonyms | Comment | Organism |
---|---|---|
NHase | - |
Rhodococcus erythropolis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Rhodococcus erythropolis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 35 | 15°C: about 60% of maximal activity, 35°C: about 55% of maximal activity, temperature profile | Rhodococcus erythropolis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 40 | 30 min, the purified enzyme is stable up to 25°C, inactivation at 40°C | Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Rhodococcus erythropolis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | pH 7.0: about 80% of maximal activity, pH 8.5: about 75% of maximal activity | Rhodococcus erythropolis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | 10°C, 30 min, purified enzyme, stable | Rhodococcus erythropolis |