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Literature summary for 4.2.1.84 extracted from

  • Sakashita, T.; Hashimoto, Y.; Oinuma, K.; Kobayashi, M.
    Transcriptional regulation of the nitrile hydratase gene cluster in Pseudomonas chlororaphis B23 (2008), J. Bacteriol., 190, 4210-4217.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.84

Activating Compound

Activating Compound Comment Organism Structure
methacrylamide induces the enzyme Pseudomonas chlororaphis

Cloned(Commentary)

Cloned (Comment) Organism
genes nhpA and nhpB encoding the alpha and beta subunits of NHase, transcriptional regulation of the nitrile hydratase gene cluster, the NHase gene cluster comprises seven genes: oxdA, amiA, nhpA, nhpB, nhpC, nhpS, and acsA, nhpR codes for a positive transcriptional regulator in the NHase gene cluster, overview Pseudomonas chlororaphis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas chlororaphis methacrylamide-induced enzyme activity, no activity in absence of methacrylamide, no or reduced activity in NhpR transcriptional regulator defective mutants ?
-
 ?
additional information Pseudomonas chlororaphis B23 methacrylamide-induced enzyme activity, no activity in absence of methacrylamide, no or reduced activity in NhpR transcriptional regulator defective mutants ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas chlororaphis
-
-
-
Pseudomonas chlororaphis B23
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 no or reduced activity in NhpR transcriptional regulator defective mutants Pseudomonas chlororaphis
23.2
-
 methacrylamide-induced enzyme activity, no activity in absence of methacrylamide Pseudomonas chlororaphis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information methacrylamide-induced enzyme activity, no activity in absence of methacrylamide, no or reduced activity in NhpR transcriptional regulator defective mutants Pseudomonas chlororaphis ?
-
 ?
additional information methacrylamide-induced enzyme activity, no activity in absence of methacrylamide, no or reduced activity in NhpR transcriptional regulator defective mutants Pseudomonas chlororaphis B23 ?
-
 ?
propionitrile + H2O
-
 Pseudomonas chlororaphis propionamide
-
 ?
propionitrile + H2O
-
 Pseudomonas chlororaphis B23 propionamide
-
 ?