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Literature summary for 4.2.1.84 extracted from
- Over-expression in Escherichia coli of a thermally stable and regio-selective nitrile hydratase from Comamonas testosteroni 5-MGAM-4D (2005), Appl. Microbiol. Biotechnol., 67, 664-670.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Comamonas testosteroni |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
x * 23000, alpha-subunit, + x * 24000, beta-subunit, calculated | Comamonas testosteroni |
| 24000 | - |
x * 23000, alpha-subunit, + x * 24000, beta-subunit, calculated | Comamonas testosteroni |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Comamonas testosteroni | Q5XPL4 | beta-subunit; expression in Escherichia coli | - |
| Comamonas testosteroni 5-MGAM-4D | Q5XPL4 | beta-subunit; expression in Escherichia coli | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxybutryronitrile + H2O | - |
Comamonas testosteroni | 3-hydroxybutyramide | yield 99% | ? |  |
| 3-hydroxybutryronitrile + H2O | - |
Comamonas testosteroni 5-MGAM-4D | 3-hydroxybutyramide | yield 99% | ? | |
| 3-hydroxypropionitrile + H2O | - |
Comamonas testosteroni | 3-hydroxypropionamide | yield 100% | ? | |
| 3-hydroxypropionitrile + H2O | - |
Comamonas testosteroni 5-MGAM-4D | 3-hydroxypropionamide | yield 100% | ? | |
| 3-hydroxyvaleronitrile + H2O | - |
Comamonas testosteroni | 3-hydroxyvaleramide | yield 99% | ? | |
| 3-hydroxyvaleronitrile + H2O | - |
Comamonas testosteroni 5-MGAM-4D | 3-hydroxyvaleramide | yield 99% | ? | |
| acrylonitrile + H2O | - |
Comamonas testosteroni | acrylamide | yield 100% | ? | |
| acrylonitrile + H2O | - |
Comamonas testosteroni 5-MGAM-4D | acrylamide | yield 100% | ? | |
| adiponitrile + 2 H2O | - |
Comamonas testosteroni | adipamide | yield 100% | ? | |
| adiponitrile + 2 H2O | - |
Comamonas testosteroni 5-MGAM-4D | adipamide | yield 100% | ? | |
| butyronitrile + H2O | - |
Comamonas testosteroni | butyramide | yield 100% | ? | |
| glycolonitrile + H2O | - |
Comamonas testosteroni | glycolamide | yield 63% | ? | |
| methacrylonitrile + H2O | - |
Comamonas testosteroni | methacrylamide | yield 100% | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23000, alpha-subunit, + x * 24000, beta-subunit, calculated | Comamonas testosteroni |
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Release 2023.1 (January 2023)
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