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Literature summary for 4.2.1.84 extracted from

  • Endo, T.; Watanabe, I.
    Nitrile hydratase of Rhodococcus sp. N-774. Purification and amino acid sequences (1989), FEBS Lett., 243, 61-64.
    View publication on PubMed
Search for more literature for 4.2.1.84

Application

Application Comment Organism
synthesis
-
 Rhodococcus sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Rhodococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28500
-
 x * 28500, alpha + x * 29000, beta, SDS-PAGE, N774 strain Rhodococcus sp.
29000
-
 x * 28500, alpha + x * 29000, beta, SDS-PAGE, N774 strain Rhodococcus sp.
70000
-
 gel filtration Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
-
-
Rhodococcus sp. N-774
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, chromatography on DEAE-Cellulofine, phenyl-Sepharose, Sephadex G-150 and octyl-Sepharose, 11.5fold purification, N-774 Rhodococcus sp.

Reaction

Reaction Comment Organism Reaction ID
an aliphatic amide = a nitrile + H2O the activity of the enzyme in intact cells increases on light irradiation and gradually decreases in the dark. For purified enzyme no differences are detected when kept in dark or light-irradiated Rhodococcus sp.
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rhodococcus sp.
1260
-
 crystallized enzyme Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
propionitrile + H2O
-
 Rhodococcus sp. propionic acid amide
-
 ?
propionitrile + H2O
-
 Rhodococcus sp. N-774 propionic acid amide
-
 ?

Subunits

Subunits Comment Organism
? x * 28500, alpha + x * 29000, beta, SDS-PAGE, N774 strain Rhodococcus sp.