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Literature summary for 4.2.1.84 extracted from

  • Tani, Y.; Kurihara, M.; Nishise, H.
    Characterization of nitrile hydratase and amidase, which are responsible for the conversion of dinitriles to mononitriles from Corynebacterium sp. (1989), Agric. Biol. Chem., 53, 3151-3158.
No PubMed abstract available
Search for more literature for 4.2.1.84

General Stability

General Stability Organism
the enzyme becomes more unstable as it becomes purer. Isovalerate, 30 mM, and caprylate stabilize effectively Corynebacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM Corynebacterium sp.
Cu2+ 1 mM CuCl2 Corynebacterium sp.
FeCl3 1 mM Corynebacterium sp.
FeSO4 1 mM Corynebacterium sp.
Hg2+ HgCl2, 0.01-1 mM Corynebacterium sp.
hydrazine 1 mM Corynebacterium sp.
KCN weak inhibition at 1 mM Corynebacterium sp.
Mn2+ MnCl2, 1 mM Corynebacterium sp.
NiCl2 1 mM Corynebacterium sp.
p-chloromercuribenzoate 1 mM Corynebacterium sp.
phenylhydrazine 1 mM Corynebacterium sp.
sulfhydryl reagents 1 mM Corynebacterium sp.
Zn2+ ZnCl2, 1 mM Corynebacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.06
-
 trans-4-Cyanocyclohexane-1-carboxylic acid
-
 Corynebacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the enzyme contains iron Corynebacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26900
-
 2 * 26900, SDS-PAGE Corynebacterium sp.
61400
-
 gel filtration Corynebacterium sp.

Organism

Organism UniProt Comment Textmining
Corynebacterium sp.
-
 C5
-
Corynebacterium sp. C5
-
 C5
-

Purification (Commentary)

Purification (Comment) Organism
chromatography on DEAE-Cellulose, DEAE-Sephacel and phenyl-Sepharose CL-4B, 130fold purification Corynebacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Corynebacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-cyanobenzoic acid + H2O
-
 Corynebacterium sp. 4-(aminocarbonyl)benzoic acid
-
 ?
4-hydroxybenzonitrile + H2O
-
 Corynebacterium sp. 4-hydroxybenzoic acid amide
-
 ?
acetonitrile + H2O
-
 Corynebacterium sp. acetamide
-
 ?
acetonitrile + H2O
-
 Corynebacterium sp. C5 acetamide
-
 ?
acrylonitrile + H2O
-
 Corynebacterium sp. 2-propenoic acid amide i.e. acrylic acid amide ?
benzonitrile + H2O
-
 Corynebacterium sp. benzoic acid amide
-
 ?
chloroacetonitrile + H2O
-
 Corynebacterium sp. chloroacetamide
-
 ?
chloroacetonitrile + H2O
-
 Corynebacterium sp. C5 chloroacetamide
-
 ?
crotononitrile + H2O 19% of the activity with propionitrile Corynebacterium sp. (E)-2-butenoic acid amide
-
 ?
isobutyronitrile + H2O
-
 Corynebacterium sp. isobutyric acid amide
-
 ?
isovaleronitrile + H2O more active than trans-4-cyanocyclohexane-1-carboxylic acid as substrate Corynebacterium sp. isovaleric acid amide
-
 ?
methacrylonitrile + H2O more active than trans-4-cyanocyclohexane-1-carboxylic acid as substrate Corynebacterium sp. methylacrylic acid amide
-
 ?
n-butyronitrile + H2O
-
 Corynebacterium sp. n-butyric acid amide
-
 ?
n-butyronitrile + H2O
-
 Corynebacterium sp. C5 n-butyric acid amide
-
 ?
n-capronitrile + H2O
-
 Corynebacterium sp. n-hexanoic acid amide
-
 ?
pivalonitrile + H2O
-
 Corynebacterium sp. 2,2-dimethylpropionic acid amide
-
 ?
propionitrile + H2O
-
 Corynebacterium sp. propionic acid amide
-
 ?
propionitrile + H2O
-
 Corynebacterium sp. C5 propionic acid amide
-
 ?
trans-4-cyanocyclohexane-1-carboxylic acid + H2O
-
 Corynebacterium sp. 4-(aminocarbonyl)cyclohexanecarboxylic acid
-
 ?
trans-4-cyanocyclohexane-1-carboxylic acid + H2O
-
 Corynebacterium sp. C5 4-(aminocarbonyl)cyclohexanecarboxylic acid
-
 ?
valeronitrile + H2O more than 3 times more active than trans-4-cyanocyclohexane-1-carboxylic acid as substrate Corynebacterium sp. n-pentanoic acid amide
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 26900, SDS-PAGE Corynebacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10
-
 pH 7.5, 10 min, stable Corynebacterium sp.
40
-
 pH 7.5, 10 min, stable Corynebacterium sp.
45
-
 pH 7.5, 10 min, 60% loss of activity Corynebacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Corynebacterium sp.