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Literature summary for 4.2.1.84 extracted from

  • Duran, R.; Chion, C.K.N.C.K.; Bigey, F.; Arnaud, A.; Galzy, P.
    The N-terminal amino acid sequences of Brevibacterium sp. R312 nitrile hydratase (1992), J. Basic Microbiol., 32, 13-19.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
x * 24000 + x * 27000, SDS-disc gel electrophoresis Arthrobacter sp.
26000
-
x * 29000, alpha, + x * 26000, beta, SDS-PAGE Rhodococcus rhodochrous
26000
-
x * 26000, alpha + x * 32000, beta, SDS-PAGE, strain 7 Rhodococcus sp.
27000
-
x * 24000 + x * 27000, SDS-disc gel electrophoresis Arthrobacter sp.
27000
-
x * 27000, alpha, + x * 27500, beta, SDS-PAGE Brevibacterium sp.
27500
-
x * 27000, alpha, + x * 27500, beta, SDS-PAGE Brevibacterium sp.
28500
-
x * 28500, alpha + x * 29000, beta, SDS-PAGE, N774 strain Rhodococcus sp.
29000
-
x * 28500, alpha + x * 29000, beta, SDS-PAGE, N774 strain Rhodococcus sp.
29000
-
x * 29000, alpha, + x * 26000, beta, SDS-PAGE Rhodococcus rhodochrous
32000
-
x * 26000, alpha + x * 32000, beta, SDS-PAGE, strain 7 Rhodococcus sp.
52000
-
-
Rhodococcus sp.
61400
-
-
Corynebacterium sp.
70000
-
N774 strain Rhodococcus sp.
90000 94000 gel filtration Brevibacterium sp.
100000
-
-
Pseudomonas chlororaphis
278000
-
strain 7 Rhodococcus sp.
420000
-
-
Arthrobacter sp.
505000 530000
-
Rhodococcus rhodochrous

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-
Brevibacterium sp.
-
-
-
Brevibacterium sp. R312
-
-
-
Corynebacterium sp.
-
-
-
Pseudomonas chlororaphis
-
-
-
Rhodococcus rhodochrous
-
-
-
Rhodococcus rhodochrous J1
-
-
-
Rhodococcus sp.
-
-
-
Rhodococcus sp. 7
-
-
-
Rhodococcus sp. N-774
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation and chromatography on Q-Sepharose, phenyl-Sepharose and Sephadex G150 Brevibacterium sp.
ammonium sulfate fractionation, chromatography on DEAE-Sephacel and phenyl-Sepharose CL-4B and gel filtration, R312 strain, 10.2fold purified Brevibacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rhodococcus rhodochrous
additional information
-
-
Corynebacterium sp.
additional information
-
-
Rhodococcus sp.
additional information
-
-
Brevibacterium sp.
additional information
-
-
Pseudomonas chlororaphis
2000
-
purified enzyme Brevibacterium sp.

Subunits

Subunits Comment Organism
? x * 28500, alpha + x * 29000, beta, SDS-PAGE, N774 strain Rhodococcus sp.
? x * 24000 + x * 27000, SDS-disc gel electrophoresis Arthrobacter sp.
? x * 29000, alpha, + x * 26000, beta, SDS-PAGE Rhodococcus rhodochrous
? x * 27000, alpha, + x * 27500, beta, SDS-PAGE Brevibacterium sp.
? x * 26000, alpha + x * 32000, beta, SDS-PAGE, strain 7 Rhodococcus sp.
tetramer
-
Rhodococcus rhodochrous
tetramer
-
Brevibacterium sp.
tetramer
-
Pseudomonas chlororaphis

pI Value

Organism Comment pI Value Maximum pI Value
Brevibacterium sp. polyacrylamide isoelectrofocusing
-
5.75