Application | Comment | Organism |
---|---|---|
synthesis | - |
Rhodococcus rhodochrous |
synthesis | - |
Pseudomonas chlororaphis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 5.7 atoms cobalt/mol enzyme, tightly bound to the protein | Rhodococcus rhodochrous | |
Co2+ | indispensable for high enzyme activity | Rhodococcus rhodochrous | |
Co2+ | 0.1% w/v, greatly increases the enzyme activity. Different Co2+ compounds increase the enzyme activity | Rhodococcus rhodochrous | |
Co2+ | enzyme of J1 strain contains cobalt | Rhodococcus rhodochrous | |
Fe2+ | at 0.1% w/v does not enhance the enzyme activity | Rhodococcus rhodochrous | |
additional information | CuSO4, CaCl2, ZnSO4, MnCl2, NaMnO4, FeCl3, FeSO4, AlCl3, BaCl2, SrCl2, NaWO4, SnCl2, BeSO4, NiCl2, PbCl2, LiCl and CoCl2 at 0.1% w/v neither inhibit nor enhance the enzyme activity | Rhodococcus rhodochrous |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
x * 29000, alpha, + x * 26000, beta, SDS-PAGE | Rhodococcus rhodochrous |
29000 | - |
x * 29000, alpha, + x * 26000, beta, SDS-PAGE | Rhodococcus rhodochrous |
505000 | 530000 | sedimentation equilibrium method, gel permeation HPLC | Rhodococcus rhodochrous |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas chlororaphis | - |
- |
- |
Rhodococcus rhodochrous | - |
- |
- |
Rhodococcus rhodochrous J1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus rhodochrous |
ammonium sulfate fractionation, chromatography on DEAE-Sephacel, octyl-Sepharose CL-4B and phenyl-Sepharose CL-4B, 4.91fold purification | Rhodococcus rhodochrous |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhodococcus rhodochrous |
additional information | - |
- |
Pseudomonas chlororaphis |
0.084 | - |
- |
Rhodococcus rhodochrous |
5.21 | - |
with the addition of 0.1% w/v CoCl2 | Rhodococcus rhodochrous |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, alpha, + x * 26000, beta, SDS-PAGE | Rhodococcus rhodochrous |