Crystallization (Comment) | Organism |
---|---|
- |
Brevibacterium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Isobutyronitrile | - |
Brevibacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.6 | - |
n-valeronitrile | pH 7, 20ºC | Brevibacterium sp. | |
8.3 | - |
n-butyronitrile | pH 7, 20ºC | Brevibacterium sp. | |
9.5 | - |
Methacrylonitrile | pH 7, 20ºC | Brevibacterium sp. | |
10.5 | - |
Propionitrile | pH 7, 20ºC | Brevibacterium sp. | |
16.7 | - |
acrylonitrile | pH 7, 20ºC | Brevibacterium sp. | |
25 | - |
Succinonitrile | pH 7, 20ºC | Brevibacterium sp. | |
30.3 | - |
Chloroacetonitrile | pH 7, 20ºC | Brevibacterium sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | contains approximately 3 g-atoms iron/mol enzyme, tightly bound to the protein | Brevibacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
x * 26000, alpha, + x * 27500, beta, SDS-PAGE | Brevibacterium sp. |
27500 | - |
x * 26000, alpha, + x * 27500, beta, SDS-PAGE | Brevibacterium sp. |
77000 | - |
gel filtration on Sephadex-G150 | Brevibacterium sp. |
85000 | - |
sedimentation equilibrium method | Brevibacterium sp. |
87000 | - |
gel filtration, HPLC | Brevibacterium sp. |
90000 | 94000 | gel filtration on Cellulofine GCL-2000sf | Brevibacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacterium sp. | - |
- |
- |
Brevibacterium sp. R312 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, chromatography on DEAE-Sephacel and phenyl-Sepharose CL-4B and gel filtration, R312 strain, 10.2fold purified | Brevibacterium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Brevibacterium sp. |
1890 | - |
- |
Brevibacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acrylonitrile + H2O | 78% of the activity with propionitrile | Brevibacterium sp. | 2-propenoic acid amide | i.e. acrylic acid amide | ? | |
adiponitrile + 2 H2O | 108% of the activity with propionitrile | Brevibacterium sp. | adipic acid amide | - |
? | |
chloroacetonitrile + H2O | 42% of the activity with propionitrile | Brevibacterium sp. | chloroacetamide | - |
? | |
chloroacetonitrile + H2O | 42% of the activity with propionitrile | Brevibacterium sp. R312 | chloroacetamide | - |
? | |
crotononitrile + H2O | 19% of the activity with propionitrile | Brevibacterium sp. | (E)-2-butenoic acid amide | - |
? | |
ethylene cyanhydrine + H2O | - |
Brevibacterium sp. | ? | - |
? | |
glutaronitrile + H2O | 38% of the activity with propionitrile | Brevibacterium sp. | ? | - |
? | |
hydroxyacetonitrile + H2O | 50% of the activity with propionitrile | Brevibacterium sp. | hydroxyacetamide | - |
? | |
malonitrile + H2O | 44% of the activity with propionitrile | Brevibacterium sp. | ? | - |
? | |
methacrylonitrile + H2O | 53% of the activity with propionitrile | Brevibacterium sp. | methylacrylic acid amide | - |
? | |
methoxyacetonitrile + H2O | - |
Brevibacterium sp. | ? | - |
? | |
n-butyronitrile + H2O | 140% of the activity with propionitrile | Brevibacterium sp. | n-butyric acid amide | - |
? | |
n-butyronitrile + H2O | 140% of the activity with propionitrile | Brevibacterium sp. R312 | n-butyric acid amide | - |
? | |
n-capronitrile + H2O | 46% of the activity with propionitrile | Brevibacterium sp. | n-hexanoic acid amide | - |
? | |
n-capronitrile + H2O | 46% of the activity with propionitrile | Brevibacterium sp. R312 | n-hexanoic acid amide | - |
? | |
pivalonitrile + H2O | 5% of the activity with propionitrile | Brevibacterium sp. | 2,2-dimethylpropionic acid amide | - |
? | |
propionitrile + H2O | - |
Brevibacterium sp. | propionic acid amide | i.e. propionamide | ? | |
propionitrile + H2O | - |
Brevibacterium sp. R312 | propionic acid amide | i.e. propionamide | ? | |
succinonitrile + H2O | 135% of the activity with propionitrile | Brevibacterium sp. | ? | - |
? | |
valeronitrile + H2O | - |
Brevibacterium sp. | n-pentanoic acid amide | - |
? | |
valeronitrile + H2O | - |
Brevibacterium sp. R312 | n-pentanoic acid amide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26000, alpha, + x * 27500, beta, SDS-PAGE | Brevibacterium sp. |
trimer | strain R312 | Brevibacterium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Brevibacterium sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0054 | - |
Isobutyronitrile | pH 7, 20ºC, competitive inhibitor | Brevibacterium sp. |