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Literature summary for 4.2.1.83 extracted from

  • Ni, B.; Zhang, Y.; Chen, D.W.; Wang, B.J.; Liu, S.J.
    Assimilation of aromatic compounds by Comamonas testosteroni: characterization and spreadability of protocatechuate 4,5-cleavage pathway in bacteria (2013), Appl. Microbiol. Biotechnol., 97, 6031-6041.
    View publication on PubMed
Search for more literature for 4.2.1.83

Cloned(Commentary)

Cloned (Comment) Organism
gene pmdE Comamonas testosteroni
gene pmdE, expression of wild-type and mutants in Escherichia coli Comamonas testosteroni

Protein Variants

Protein Variants Comment Organism
additional information generation of several pmdE gene truncated mutants, lacking residues 6 to 226, 32 to 296, or 105 to 215, overview. A pmdE knock out mutant loses the ability to grow on vanillate, 3-hydroxybenzoate, and 4-hydroxybenzoate as carbon sources. This ability to grow on vanillate, 3-hydroxybenzoate, and 4-hydroxybenzoate is restored by genetic complementation Comamonas testosteroni

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni D0IZN8 gene pmdE
-
Comamonas testosteroni Q93PS9 gene pmdE
-
Comamonas testosteroni CNB-1 Q93PS9 gene pmdE
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Comamonas testosteroni CNB-2 D0IZN8 gene pmdE
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Source Tissue

Source Tissue Comment Organism Textmining
culture condition:3-hydroxybenzoate-grown cell
-
 Comamonas testosteroni
-
culture condition:4-hydroxybenzoate-grown cell
-
 Comamonas testosteroni
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culture condition:gentisate-grown cell
-
 Comamonas testosteroni
-
culture condition:phenol-grown cell
-
 Comamonas testosteroni
-
culture condition:vanillate-grown cell
-
 Comamonas testosteroni
-
additional information strain CNB-1 grows on phenol, gentisate, vanillate, 3-hydroxybenzoate, and 4-hydroxybenzoate as carbon and energy sources, but does not grow on resorcinol, 2,4-dihydroxybenzoate, 4-cresol, and phenylacetate Comamonas testosteroni
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-4-carboxy-hexa-2,4-dienedioate 4-oxalomesaconate hydratase uses only enol-OMA as substrate Comamonas testosteroni 4-carboxy-4-hydroxy-2-oxoadipate + H2O
-
 ?
2-hydroxy-4-carboxy-hexa-2,4-dienedioate 4-oxalomesaconate hydratase uses only enol-OMA as substrate Comamonas testosteroni CNB-2 4-carboxy-4-hydroxy-2-oxoadipate + H2O
-
 ?
2-hydroxy-4-carboxy-hexa-2,4-dienedioate 4-oxalomesaconate hydratase uses only enol-OMA as substrate Comamonas testosteroni CNB-1 4-carboxy-4-hydroxy-2-oxoadipate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
OMA
-
 Comamonas testosteroni
PmdE
-
 Comamonas testosteroni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Comamonas testosteroni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at, about Comamonas testosteroni

pI Value

Organism Comment pI Value Maximum pI Value
Comamonas testosteroni sequence calculation
-
 5.81
Comamonas testosteroni isoelectric focusing
-
 6.23

General Information

General Information Comment Organism
evolution comparison of gene clusters of the protocatechuate 4,5-cleavage pathway in bacterial strains, overview Comamonas testosteroni
malfunction a pmdE knock out mutant loses the ability to grow on vanillate, 3-hydroxybenzoate, and 4-hydroxybenzoate as carbon sources. This ability to grow on vanillate, 3-hydroxybenzoate, and 4-hydroxybenzoate is restored by genetic complementation Comamonas testosteroni
metabolism the enzyme is active in the protocatechuate 4,5-cleavage pathway. Gene pmdU encodes an 4-oxalomesaconate tautomerase and catalyzes conversion of 4-oxalomesaconate-keto into 4-oxalomesaconate-enol, which is the only substrate of 4-oxalomesaconate hydratase. Pathway overview Comamonas testosteroni
physiological function the enzyme in strain CNB-1 is essential for growth on phenol, gentisate, vanillate, 3-hydroxybenzoate, and 4-hydroxybenzoate as carbon and energy sources Comamonas testosteroni